Department of Biochemistry and Biotechnology, Universitat Rovira i Virgili, Reus, Spain.
Institut D'Investigació Sanitària Pere Virgili (IISPV), Hospital Universitari de Sant Joan de Reus, Reus, Spain.
Front Endocrinol (Lausanne). 2022 Apr 28;13:804455. doi: 10.3389/fendo.2022.804455. eCollection 2022.
An altered gut microbiota has been associated with insulin resistance, a metabolic dysfunction consisting of cellular insulin signaling impairment. The aim of the present study is to determine the taxonomic and functional fecal microbiota signatures associated with HOMA-IR index in a population with high cardiovascular risk.
A total of 279 non-diabetic individuals (55-75 years aged) with overweight/obesity and metabolic syndrome were stratified according to tertiles of HOMA-IR index. Blood biochemical parameters, anthropometric measurements and fecal samples were collected at baseline. Fecal microbial DNA extraction, 16S amplicon sequencing and bioinformatics analysis were performed.
, and UCG-002 were negatively associated with HOMA-IR index, whereas predicted total functional abundances revealed gut metabolic modules mainly linked to amino acid degradation. , UCG-003, were positively associated with HOMA-IR index, whereas predicted total functional abundances revealed gut metabolic modules mainly linked to saccharide degradation. These bacteria contribute differentially to the gut metabolic modules, being the degree of contribution dependent on insulin resistance. Both taxa and gut metabolic modules negatively associated to HOMA-IR index were linked to mechanisms involving sulfate reducing bacteria, improvement of intestinal gluconeogenesis and production of acetate. Furthermore, both taxa and gut metabolic modules positively associated to HOMA-IR index were linked to production and mechanisms of action of butyrate.
Specific taxonomic and functional fecal microbiota signatures associated with insulin resistance were identified in a non-diabetic population with overweight/obesity at high cardiovascular risk. These findings suggest that tailoring therapies based on specific fecal microbiota profiles could be a potential strategy to improve insulin sensitivity.
肠道微生物群的改变与胰岛素抵抗有关，胰岛素抵抗是一种代谢功能障碍，包括细胞胰岛素信号受损。本研究旨在确定与高心血管风险人群中 HOMA-IR 指数相关的粪便微生物群的分类和功能特征。
共纳入 279 名非糖尿病超重/肥胖和代谢综合征患者（年龄 55-75 岁），根据 HOMA-IR 指数的三分位数进行分层。在基线时采集血液生化参数、人体测量学测量值和粪便样本。进行粪便微生物 DNA 提取、16S 扩增子测序和生物信息学分析。
与 HOMA-IR 指数呈负相关的是、和 UCG-002，而预测的总功能丰度显示与氨基酸降解有关的肠道代谢模块。与 HOMA-IR 指数呈正相关的是、UCG-003 和，而预测的总功能丰度显示与糖降解有关的肠道代谢模块。这些细菌对肠道代谢模块的贡献不同，其贡献程度取决于胰岛素抵抗。与 HOMA-IR 指数呈负相关的细菌与涉及硫酸盐还原菌、改善肠道糖异生和产生乙酸的机制有关。此外，与 HOMA-IR 指数呈正相关的细菌与丁酸盐的产生和作用机制有关。
在超重/肥胖且具有高心血管风险的非糖尿病人群中，确定了与胰岛素抵抗相关的特定粪便微生物群的分类和功能特征。这些发现表明，基于特定粪便微生物群谱的靶向治疗可能是提高胰岛素敏感性的一种潜在策略。
