Solaberrieta Ignacio, Jiménez Alfonso, Garrigós María Carmen
Department of Analytical Chemistry, Nutrition & Food Sciences, University of Alicante, San Vicente del Raspeig, ES-03690 Alicante, Spain.
Antioxidants (Basel). 2022 May 26;11(6):1058. doi: 10.3390/antiox11061058.
skin (AVS) is a major by-product of Aloe processing plants all over the world. In this study, response surface methodology was used to optimize microwave-assisted extraction (MAE) of bioactive compounds from AVS. The influence of extraction parameters, such as ethanol concentration (%Et), extraction temperature (T), time (t) and solvent volume (V), on extraction yield (Y), total phenolic content (TPC), antioxidant activity (DPPH and FRAP methods) and aloin content, was studied. Optimum extraction conditions were determined as 80% ethanol, 80 °C, 36.6 min and 50 mL and optimized extracts showed interesting contents of polyphenols and antioxidant performance. The phenolic profile was determined by HPLC-DAD/MS and some major phenolic compounds, such as aloin A, aloin B, aloesin, aloe-emodin, aloeresin D, orientin, cinnamic acid and chlorogenic acid, were quantified while eight other compounds were tentatively identified. Moreover, structural and thermal properties were studied by FTIR and TGA analyses, respectively. The obtained results suggested the potential of AVS as a promising source of bioactive compounds, thus increasing the added value of this agricultural waste.
芦荟皮（AVS）是世界各地芦荟加工厂的一种主要副产品。在本研究中，采用响应面法优化了从AVS中微波辅助提取（MAE）生物活性化合物的工艺。研究了乙醇浓度（%Et）、提取温度（T）、时间（t）和溶剂量（V）等提取参数对提取率（Y）、总酚含量（TPC）、抗氧化活性（DPPH和FRAP法）和芦荟苷含量的影响。确定最佳提取条件为80%乙醇、80℃、36.6分钟和50毫升，优化后的提取物显示出有趣的多酚含量和抗氧化性能。通过HPLC-DAD/MS测定酚类成分，对一些主要酚类化合物，如芦荟苷A、芦荟苷B、芦荟素、芦荟大黄素、芦荟树脂D、木犀草素、肉桂酸和绿原酸进行了定量，同时初步鉴定了其他八种化合物。此外，分别通过FTIR和TGA分析研究了结构和热性能。所得结果表明AVS作为生物活性化合物的潜在来源具有潜力，从而提高了这种农业废弃物的附加值。
