Fu Yuying, Zhang Lishan, Song Huanhuan, Liao Junyan, Lin Li, Jiang Wenjia, Wu Xiaoyun, Wang Guibin
School of Safety and Environment, Fujian Chuanzheng Communications College, Fuzhou 350007, China.
Fujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring, School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Antibiotics (Basel). 2022 Jun 23;11(7):841. doi: 10.3390/antibiotics11070841.
The antibiotic resistance of is becoming increasingly prevalent, and thus novel antimicrobial strategies are being sought. Lysine acylation has been demonstrated to play an important role in bacterial physiological functions, while its role in bacterial antibiotic resistance remains largely unclear. In this study, we investigated the lysine acetylation and succinylation profiles of strain EIB202 using affinity antibody purification combined with LC-MS/MS. A total of 1511 lysine-acetylation sites were identified on 589 proteins, and 2346 lysine-succinylation sites were further identified on 692 proteins of this pathogen. Further bioinformatic analysis showed that both post-translational modifications (PTMs) were enriched in the tricarboxylic acid (TCA) cycle, pyruvate metabolism, biosynthesis, and carbon metabolism. In addition, 948 peptides of 437 proteins had overlapping associations with multiple metabolic pathways. Moreover, both acetylation and succinylation were found in many antimicrobial resistance (AMR) proteins, suggesting their potentially vital roles in antibiotic resistance. In general, our work provides insights into the acetylome and succinylome features responsible for the antibiotic resistance mechanism of , and the results may facilitate future investigations into the pathogenesis of this bacterium.
细菌的抗生素耐药性日益普遍，因此人们正在寻找新的抗菌策略。赖氨酸酰化已被证明在细菌生理功能中起重要作用，但其在细菌抗生素耐药性中的作用仍 largely 不清楚。在本研究中，我们使用亲和抗体纯化结合 LC-MS/MS 研究了 EIB202 菌株的赖氨酸乙酰化和琥珀酰化谱。在该病原体的 589 种蛋白质上共鉴定出 1511 个赖氨酸乙酰化位点，在 692 种蛋白质上进一步鉴定出 2346 个赖氨酸琥珀酰化位点。进一步的生物信息学分析表明，这两种翻译后修饰（PTMs）都富集于三羧酸（TCA）循环、丙酮酸代谢、生物合成和碳代谢中。此外，437 种蛋白质的 948 个肽段与多种代谢途径有重叠关联。此外，在许多抗微生物耐药性（AMR）蛋白质中都发现了乙酰化和琥珀酰化，表明它们在抗生素耐药性中可能起着至关重要的作用。总体而言，我们的工作为负责该细菌抗生素耐药机制的乙酰化组和琥珀酰化组特征提供了见解，结果可能有助于未来对该细菌发病机制的研究。
