Department of Biotechnology, University of Chemistry and Technology, Prague, Czech Republic.
Research Centre Řež, Husinec, Czech Republic.
PLoS One. 2022 Aug 10;17(8):e0272844. doi: 10.1371/journal.pone.0272844. eCollection 2022.
Green methods have become vital for sustainable development of the scientific and commercial sphere; however, they can bring new challenges, including the need for detailed characterization and elucidation of efficacy of their products. In this study, green method of silver nanoparticles (AgNPs) production was employed using an extract from grapevine canes. The aim of the study was to contribute to the knowledge about biosynthesized AgNPs by focusing on elucidation of their antifungal efficiency based on their size and/or hypothesized synergy with bioactive substances from Vitis vinifera cane extract. The antifungal activity of AgNPs capped and stabilized with bioactive compounds was tested against the opportunistic pathogenic yeast Candida albicans. Two dispersions of nanoparticles with different morphology (characterized by SEM-in-STEM, DLS, UV-Vis, XRD, and AAS) were prepared by modification of reaction conditions suitable for economical production and their long-term stability monitored for six months was confirmed. The aims of the study included the comparison of the antifungal effect against suspension cells and biofilm of small monodisperse AgNPs with narrow size distribution and large polydisperse AgNPs. The hypothesis of synergistic interaction of biologically active molecules from V. vinifera extracts and AgNPs against both cell forms were tested. The interactions of all AgNPs dispersions with the cell surface and changes in cell morphology were imaged using SEM. All variants of AgNPs dispersions were found to be active against suspension and biofilm cells of C. albicans; nevertheless, surprisingly, larger polydisperse AgNPs were found to be more effective. Synergistic action of nanoparticles with biologically active extract compounds was proven for biofilm cells (MBIC80 20 mg/L of polydisperse AgNPs in extract), while isolated nanoparticles suspended in water were more active against suspension cells (MIC 20 mg/L of polydisperse AgNPs dispersed in water). Our results bring new insight into the economical production of AgNPs with defined characteristics, which were proven to target a specific mode of growth of significant pathogen C. albicans.
绿色方法已成为科学和商业领域可持续发展的关键；然而，它们也带来了新的挑战，包括需要详细描述和阐明其产品的功效。在本研究中，使用葡萄藤提取物采用绿色方法生产了银纳米粒子（AgNPs）。本研究的目的是通过关注根据其大小和/或与葡萄藤提取物中的生物活性物质的假设协同作用来阐明生物合成 AgNPs 的知识，为其做出贡献。用生物活性化合物稳定的 AgNPs 的抗真菌活性针对机会致病酵母白色念珠菌进行了测试。通过修饰适合经济生产的反应条件制备了两种具有不同形态的纳米粒子分散体（通过 SEM-STEM、DLS、UV-Vis、XRD 和 AAS 进行表征），并确认了其长达六个月的长期稳定性。本研究的目的包括比较具有窄尺寸分布的小单分散 AgNPs 和具有大的多分散性的 AgNPs 的悬浮细胞和生物膜的抗真菌效果。测试了从 V. vinifera 提取物中提取的生物活性分子与 AgNPs 对两种细胞形式的协同作用的假设。使用 SEM 对所有 AgNPs 分散体与细胞表面的相互作用以及细胞形态的变化进行了成像。发现所有 AgNPs 分散体对悬浮液和生物膜细胞的白色念珠菌均具有活性；然而，令人惊讶的是，更大的多分散性 AgNPs 更有效。证明了纳米粒子与生物活性提取物化合物的协同作用对生物膜细胞有效（生物膜细胞中分散有生物活性提取物的多分散性 AgNPs 的 MBIC80 为 20mg/L），而分散在水中的单独纳米粒子对悬浮细胞的活性更高（分散在水中的多分散性 AgNPs 的 MIC 为 20mg/L）。我们的结果为具有特定特性的 AgNPs 的经济生产提供了新的见解，这些 AgNPs 被证明针对重要病原体白色念珠菌的特定生长模式。
