Spinoso-Castillo José Luis, Bello-Bello Jericó Jabín
Colegio de Postgraduados-Campus Córdoba, Amatlán de los Reyes, Veracruz, Mexico.
CONACYT-Colegio de Postgraduados-Campus Córdoba, Amatlán de los Reyes, Veracruz, Mexico.
Methods Mol Biol. 2022;2527:223-235. doi: 10.1007/978-1-0716-2485-2_16.
Somatic embryogenesis (SE) serves as a key biological model for studying cell totipotency and the ontogenic processes of zygotic embryogenesis in plants. The SE process, under in vitro conditions, can be induced from different sources of explant cultivated in a culture medium with plant growth regulators (PGR) or by subjecting tissues to abiotic stress treatments. Somatic embryogenesis, in plant tissue culture (PTC), is a multifactorial event. The use of PGR, particularly auxins, is an important factor during induction. However, in vitro abiotic stress treatments are physiologically, biochemically, and genetically relevant and should be further studied.
体细胞胚胎发生（SE）是研究植物细胞全能性和合子胚胎发生个体发育过程的关键生物学模型。在体外条件下，SE过程可以从在含有植物生长调节剂（PGR）的培养基中培养的不同外植体来源诱导产生，或者通过使组织经受非生物胁迫处理来诱导。在植物组织培养（PTC）中，体细胞胚胎发生是一个多因素事件。PGR的使用，尤其是生长素，是诱导过程中的一个重要因素。然而，体外非生物胁迫处理在生理、生化和遗传方面都具有相关性，应进一步研究。
