State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
Int J Mol Sci. 2022 Aug 30;23(17):9873. doi: 10.3390/ijms23179873.
The infant gut microbiota is critical for promoting and maintaining early-life health. The study aimed to analyze the composition of sIgA-coated and sIgA-uncoated bacterial communities at genus level and lactobacilli and bifidobacterial communities at species level in human breast milk (HBM) and infant and maternal feces. Eleven pregnant women were recruited successfully. HBM; infant feces during colostrum, transition, and mature stages; and maternal feces within the mature stage were collected. sIgA-coated and sIgA-uncoated bacteria were separated with magnetic-activated cell sorting. Then, 16S rRNA sequencing, bifidobacterial gene sequencing, and lactobacilli gene sequencing were performed to analyze the bacterial community. PCoA revealed that the compositions of sIgA-coated and sIgA-uncoated bacteria were different among HBM and infant and maternal feces. Higher relative abundance of sIgA-uncoated was found in the three lactation stages in infant feces compared to the corresponding HBM, and a higher relative abundance of sIgA-uncoated was found in maternal feces compared to HBM and infant feces. For bifidobacterial community, sIgA-coated and sIgA-uncoated subsp. and was dominant in infant feces and maternal feces, respectively. The relative abundance of sIgA-uncoated subsp. was significantly higher in infant feces compared to that in maternal feces. For the community, . and . were dominant in infant and maternal feces, respectively. HBM and infant and maternal feces showed distinct diversity and composition of both sIgA-coated and sIgA-uncoated bacteria at genus level. Infant and maternal feces showed similar composition of at species level. The same species could be detected both in sIgA-coated and -uncoated form. This article provided deeper understanding on the microbiota profile in HBM and infant and maternal feces.
人乳中分泌型免疫球蛋白 A（sIgA）包裹和未包裹细菌群落及乳杆菌和双歧杆菌群落组成在属水平和种水平上的分析。
成功招募了 11 名孕妇。收集人乳（HBM）；婴儿初乳、过渡和成熟阶段的粪便；以及成熟阶段的母亲粪便。使用磁激活细胞分选法分离 sIgA 包裹和未包裹细菌。然后，进行 16S rRNA 测序、双歧杆菌基因测序和乳杆菌基因测序，以分析细菌群落。PCoA 显示 HBM 和婴儿及母亲粪便中的 sIgA 包裹和未包裹细菌组成不同。与相应的 HBM 相比，婴儿粪便的三个泌乳阶段 sIgA 未包裹的相对丰度更高，与 HBM 和婴儿粪便相比，母亲粪便 sIgA 未包裹的相对丰度更高。双歧杆菌群落中，sIgA 包裹和未包裹的双歧杆菌亚种和在婴儿粪便和母亲粪便中分别占优势。与母亲粪便相比，婴儿粪便中 sIgA 未包裹的亚种的相对丰度显著更高。在 群落中，分别在婴儿和母亲粪便中占优势。HBM 和婴儿及母亲粪便在属水平上均表现出 sIgA 包裹和未包裹细菌的多样性和组成明显不同。婴儿和母亲粪便在种水平上具有相似的组成。在 sIgA 包裹和未包裹形式中都可以检测到相同的 种。本文深入了解了 HBM 和婴儿及母亲粪便中的微生物组特征。
