Collaborative Innovation Center of Advanced Microstructures, National Laboratory of Solid State Microstructure, Key Laboratory of Intelligent Optical Sensing and Manipulation, Ministry of Education, Department of Physics, Nanjing University, Nanjing 210093, China.
Oujiang Laboratory (Zhejiang Lab for Regenerative Medicine, Vision and Brain Health), Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou 325001, China.
Int J Mol Sci. 2022 Sep 1;23(17):9987. doi: 10.3390/ijms23179987.
Glue-type bio-adhesives are in high demand for many applications, including hemostasis, wound closure, and integration of bioelectronic devices, due to their injectable ability and in situ adhesion. However, most glue-type bio-adhesives cannot be used for short-term tissue adhesion due to their weak instant cohesion. Here, we show a novel glue-type bio-adhesive based on the phase separation of proteins and polysaccharides by functionalizing polysaccharides with dopa. The bio-adhesive exhibits increased adhesion performance and enhanced phase separation behaviors. Because of the cohesion from phase separation and adhesion from dopa, the bio-adhesive shows excellent instant and long-term adhesion performance for both organic and inorganic substrates. The long-term adhesion strength of the bio-glue on wet tissues reached 1.48 MPa (shear strength), while the interfacial toughness reached ~880 J m. Due to the unique phase separation behaviors, the bio-glue can even work normally in aqueous environments. At last, the feasibility of this glue-type bio-adhesive in the adhesion of various visceral tissues in vitro was demonstrated to have excellent biocompatibility. Given the convenience of application, biocompatibility, and robust bio-adhesion, we anticipate the bio-glue may find broad biomedical and clinical applications.
胶型生物粘合剂由于其可注射性和原位粘附性，在许多应用中都有很高的需求，包括止血、伤口闭合和生物电子设备的集成。然而，由于大多数胶型生物粘合剂的瞬间内聚强度较弱，因此不能用于短期组织粘附。在这里，我们展示了一种基于多巴功能化多糖的蛋白质和多糖相分离的新型胶型生物粘合剂。生物粘合剂表现出增强的粘附性能和增强的相分离行为。由于相分离的内聚和多巴的粘附，生物粘合剂在有机和无机基底上均表现出优异的即时和长期粘附性能。生物胶在湿组织上的长期粘附强度达到 1.48 MPa（剪切强度），而界面韧性达到约 880 J m。由于独特的相分离行为，生物胶甚至可以在水相环境中正常工作。最后，体外粘附各种内脏组织的实验证明了这种胶型生物粘合剂具有良好的生物相容性。鉴于其应用方便、生物相容性和强大的生物粘附性，我们预计该生物胶可能会在生物医学和临床应用中得到广泛应用。
