Department of Agro-Environmental Sciences, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan.
Department of Pharmacognosy, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt.
Molecules. 2022 Sep 17;27(18):6076. doi: 10.3390/molecules27186076.
L. () has a long history of edible and medicinal uses. In this study, the biological activities of the extracts, chromatographic fractions, and hibiscus acid obtained from were evaluated for their potential bioactivities. Their ability to promote extracellular matrix synthesis in skin fibroblasts was evaluated by enzyme-linked immunosorbent assays. Their anti-inflammatory activity was evaluated in a nitric oxide (NO)-Griess inflammatory experiment. Furthermore, hibiscus acid was found to have a strong anti-oxidative stress effect through the establishment of an oxidative stress model induced by hydrogen peroxide. Several assays indicated that hibiscus acid treatment can effectively reduce extracellular adenosine triphosphate (ATP) secretion and carbonyl protein production, as well as maintain a high level of reduced/oxidized glutathione (GSH/GSSG) in skin cells, thus providing a possible mechanism by which hibiscus acid can counter antioxidative stress. The present study is the first to explore the reversing skin aging potential and the contributory component of .
山茶花具有悠久的食用和药用历史。在这项研究中，评估了从山茶花中提取的提取物、色谱馏分和芙蓉酸的生物活性，以评估它们的潜在生物活性。通过酶联免疫吸附试验评估它们促进皮肤成纤维细胞外基质合成的能力。在一氧化氮（NO）-Griess 炎症实验中评估其抗炎活性。此外，通过建立由过氧化氢诱导的氧化应激模型，发现芙蓉酸具有很强的抗氧化应激作用。几项测定表明，芙蓉酸处理可以有效减少细胞外三磷酸腺苷（ATP）的分泌和羰基蛋白的产生，并保持皮肤细胞中还原/氧化型谷胱甘肽（GSH/GSSG）的高水平，从而为芙蓉酸提供了一种可能的机制，可以对抗抗氧化应激。本研究首次探索了山茶花的逆转皮肤衰老潜力及其组成成分。
