Department of Biochemistry and Biomedical Sciences, McMaster University, 1280 Main Street West, Hamilton, Ontario, L8S 4K1, Canada.
Department of Engineering Physics, McMaster University, 1280 Main Street West, Hamilton, Ontario, L8S 4K1, Canada.
Angew Chem Int Ed Engl. 2022 Dec 12;61(50):e202212496. doi: 10.1002/anie.202212496. Epub 2022 Oct 27.
Nucleic acids are remarkable molecules. In addition to Watson-Crick base pairing, the different structural motifs of these molecules can bind non-nucleic acid targets or catalyze chemical reactions. Additionally, nucleic acids are easily modified with different molecules or functional groups. These properties make nucleic acids, particularly DNA, ideally suited for use in electrochemical biosensors, both as biorecognition elements and redox reporter probes. In this Minireview, we will review the historical evolution of nucleic acids as probes in electrochemical biosensors. We will then review the specific examples of nucleic-acid-based biosensors that have been evaluated for clinical use in the areas of infectious disease, cancer, or cardiovascular health.
核酸是一种非凡的分子。除了 Watson-Crick 碱基配对外，这些分子的不同结构基序可以与非核酸靶标结合或催化化学反应。此外，核酸很容易用不同的分子或官能团进行修饰。这些特性使核酸（尤其是 DNA）非常适合用作电化学生物传感器中的生物识别元件和氧化还原报告探针。在这篇综述中，我们将回顾核酸作为电化学生物传感器中的探针的历史演变。然后，我们将回顾基于核酸的生物传感器的具体实例，这些传感器已在传染病、癌症或心血管健康领域的临床应用中进行了评估。
