Önder Sercan, Tonguç Muhammet, Erbaş Sabri, Önder Damla, Mutlucan Murat
Department of Agricultural Biotechnology, Faculty of Agriculture, Isparta University of Applied Sciences, Isparta, 32200, Türkiye.
Department of Field Crops, Faculty of Agriculture, Isparta University of Applied Sciences, Isparta, 32200, Türkiye.
Plant Physiol Biochem. 2022 Dec 1;192:20-34. doi: 10.1016/j.plaphy.2022.09.032. Epub 2022 Oct 3.
Oil-bearing rose is a very valuable member of the Rosa genus. Despite the importance of oil-bearing rose, metabolic changes during flower development are not well understood. Thus, the objective of this study was to investigate the changes in phenological, primary and secondary metabolites and their interactions at five developmental stages of oil-bearing rose. Flower width, flower and petal fresh weights, petal area and petal relative water content increased from bud stage to blooming stage, while flower length and sepal area increased only at early stages. Thirty-seven essential oil components were identified at different stages of petal development and nonadecane, β-citronellol and n-heneicosane were the prevalent essential oil components regardless of stage. Sixteen fatty acids were identified and the amount of saturated fatty acids was higher than the mono and polyunsaturated fatty acids in all developmental stages. Eight organic acids were detected in petals and four of them (tartaric, malic, citric and succinic acids) showed significant changes, and total organic acids content decreased during flower development. Catechin and epicatechin were the most abundant phenolic compounds in petals. While total phenolic, flavonoid and free amino acids contents decreased during flower development, total free fatty acids content increased, but was not significant between the developmental stages. Correlation analysis between phenological traits and some metablolites revealed 20 significant correlations and 11 of which were positive. Results showed that flower development stages had significant effects on metabolite content and quality of products obtained, and significant shifts in metabolite type and content occurred at flower development stages III and IV.
油用玫瑰是蔷薇属中一种非常有价值的植物。尽管油用玫瑰很重要，但人们对其花发育过程中的代谢变化了解并不充分。因此，本研究的目的是调查油用玫瑰五个发育阶段的物候、初级和次级代谢产物的变化及其相互作用。花宽、花和花瓣鲜重、花瓣面积和花瓣相对含水量从花蕾期到盛开期增加，而花长和萼片面积仅在早期增加。在花瓣发育的不同阶段鉴定出37种精油成分，无论处于哪个阶段，十九烷、β-香茅醇和二十一烷都是主要的精油成分。鉴定出16种脂肪酸，在所有发育阶段饱和脂肪酸的含量均高于单不饱和脂肪酸和多不饱和脂肪酸。在花瓣中检测到8种有机酸，其中4种（酒石酸、苹果酸、柠檬酸和琥珀酸）表现出显著变化，花发育过程中总有机酸含量下降。儿茶素和表儿茶素是花瓣中含量最丰富的酚类化合物。虽然花发育过程中总酚、黄酮和游离氨基酸含量下降，但总游离脂肪酸含量增加，但在发育阶段之间差异不显著。物候性状与一些代谢物之间的相关性分析显示有20个显著相关性，其中11个为正相关。结果表明，花发育阶段对所获得产品的代谢物含量和品质有显著影响，在花发育的III期和IV期代谢物类型和含量发生了显著变化。
