一种用于测量药物对精子活力影响的改良跨膜迁移方法。
A modified trans-membrane migration method for measuring the effect of drugs on sperm motility.
作者信息
Raoof N T, Pearson R M, Turner P
机构信息
Department of Clinical Pharmacology, St Bartholomew's Hospital Medical College, London.
出版信息
Br J Clin Pharmacol. 1987 Sep;24(3):319-21. doi: 10.1111/j.1365-2125.1987.tb03175.x.
Abstract
- The trans-membrane migration ratio (TMMR) technique is a simple method for quantitating and comparing the effect of drugs on sperm motility. 2. The ultrafilter membrane (Nucleopore) used allows mixing between the solutions on either side resulting in 21-fold dilution of a drug initially mixed with semen. 3. The concentration of drug originally mixed with semen does not remain constant over the incubation time. 4. The phosphate buffer saline (PBS) in the lower chamber should be replaced by PBS containing the same concentration of drug as in the upper chamber so that a homogenous system is established throughout the incubation. 5. Reports of previous studies using this technique may have underestimated the effect of drugs on sperm motility.
摘要
- 跨膜迁移率（TMMR）技术是一种用于定量和比较药物对精子活力影响的简单方法。2. 所使用的超滤膜（核孔膜）允许两侧溶液混合，导致最初与精液混合的药物稀释21倍。3. 最初与精液混合的药物浓度在孵育期间不会保持恒定。4. 下腔室中的磷酸盐缓冲盐水（PBS）应替换为含有与上腔室相同浓度药物的PBS，以便在整个孵育过程中建立均匀系统。5. 以前使用该技术的研究报告可能低估了药物对精子活力的影响。
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