通过DNA杂交法测定金黄色葡萄球菌对氨基糖苷类抗生素的耐药性。
Determination of aminoglycoside resistance in Staphylococcus aureus by DNA hybridization.
作者信息
Dickgiesser N, Kreiswirth B N
出版信息
Antimicrob Agents Chemother. 1986 May;29(5):930-2. doi: 10.1128/AAC.29.5.930.
Abstract
A method is described for identification of the genes conferring aminoglycoside resistance in Staphylococcus aureus by dot-blot and Southern blot techniques. As radioactive probes, fragments of plasmids pAT48, pUBH2, and pH13, carrying the genes for an aminocyclitol-3'-phosphotransferase, an aminocyclitol-4'-adenylyltransferase, and an aminocyclitol-2''-phosphotransferase-aminocyclitol-6'-acetyltransferase, respectively, were used.
摘要
描述了一种通过斑点印迹和Southern印迹技术鉴定金黄色葡萄球菌中赋予氨基糖苷抗性的基因的方法。作为放射性探针，分别使用了携带氨基环醇-3'-磷酸转移酶、氨基环醇-4'-腺苷酸转移酶和氨基环醇-2''-磷酸转移酶-氨基环醇-6'-乙酰转移酶基因的质粒pAT48、pUBH2和pH13的片段。
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