Efimov V A, Buryakova A A, Dubey I Y, Polushin N N, Chakhmakhcheva O G
Nucleic Acids Res. 1986 Aug 26;14(16):6525-40. doi: 10.1093/nar/14.16.6525.
An effective procedure for the synthesis of oligonucleotides by the phosphotriester method has been developed. The procedure is based on the use of phosphate protecting groups enabling O-nucleophilic intramolecular catalysis in the reaction of internucleotide bond formation under the action of arylsulfonyl chlorides and their derivatives. Using this new procedure, the time needed to perform one elongation step on polymer support is 7-8 min. The effectiveness of the methodology has been demonstrated in the synthesis of many oligodeoxyribonucleotides of different length with high yields.
已开发出一种通过磷酸三酯法合成寡核苷酸的有效方法。该方法基于使用磷酸保护基团，在芳基磺酰氯及其衍生物的作用下，在核苷酸间键形成反应中实现O-亲核分子内催化。使用这种新方法，在聚合物载体上进行一个延伸步骤所需的时间为7-8分钟。该方法的有效性已在合成许多不同长度的寡脱氧核糖核苷酸中得到证明，且产率很高。
