Osawa Motoyasu, Handa Osamu, Fukushima Shinya, Matsumoto Hiroshi, Umegaki Eiji, Inoue Ryo, Naito Yuji, Shiotani Akiko
Department of Internal Medicine, Division of Gastroenterology, Kawasaki Medical School, 577 Matsushima, Kurashiki, Okayama 701-0192, Japan.
Faculty of Agriculture, Setsunan University, 45-1 Nagaotoge-cho, Hirakata, Osaka 573-0101, Japan.
J Clin Biochem Nutr. 2023 Jul;73(1):77-83. doi: 10.3164/jcbn.22-86. Epub 2023 Apr 14.
Compositional changes in the microbiota are associated with various inflammatory diseases, including ulcerative colitis (UC). Aim: This study aimed to investigate the mucosa-associated microbiota (MAM) in patients with UC and its difference related with disease activity and classification. Brush samples were collected from the terminal ileum and sigmoid colon during endoscopic procedures. The microbiota of samples was profiled using the Illumina MiSeq platform. The V3-V4 regions of the gene encoding 16S rRNA (460 bp) were amplified using PCR. Fifty UC patients and twenty healthy controls were enrolled. UC patients displayed significantly reduced α-diversity in both the ileum and sigmoid colon compared to controls. A difference in β-diversity in the unweighted analysis was observed between the two groups. The abundance of and was significantly higher and that of , and was significantly lower in the ileum of UC patients than in controls. The abundance of in the ileum was significantly lower in left-sided colitis and pancolitis patients than in proctitis patients and lower in patients with highly severe disease activity than with mild disease activity. The reduction in abundance of butyric acid-producing bacteria, especially , in ileal MAM may play an important role in the pathophysiology of UC.
微生物群的组成变化与包括溃疡性结肠炎（UC）在内的各种炎症性疾病相关。目的：本研究旨在调查UC患者的黏膜相关微生物群（MAM）及其与疾病活动度和分类的差异。在内镜检查过程中从回肠末端和乙状结肠采集刷检样本。使用Illumina MiSeq平台对样本的微生物群进行分析。采用PCR扩增编码16S rRNA（460 bp）的基因的V3 - V4区域。招募了50名UC患者和20名健康对照。与对照组相比，UC患者在回肠和乙状结肠中的α多样性均显著降低。在两组之间未加权分析中观察到β多样性存在差异。UC患者回肠中 和 的丰度显著高于对照组，而 、 和 的丰度显著低于对照组。左侧结肠炎和全结肠炎患者回肠中 的丰度显著低于直肠炎患者，且疾病活动度高的患者低于疾病活动度轻的患者。回肠MAM中产生丁酸的细菌，尤其是 的丰度降低可能在UC的病理生理学中起重要作用。
