用为64K CP/M和MS-DOS IBM兼容微型计算机编写的BASIC程序组装重叠DNA序列。
Assembly of overlapping DNA sequences by a program written in BASIC for 64K CP/M and MS-DOS IBM-compatible microcomputers.
作者信息
Johnston R E, Mackenzie J M, Dougherty W G
出版信息
Nucleic Acids Res. 1986 Jan 10;14(1):517-27. doi: 10.1093/nar/14.1.517.
Abstract
The SEQALIGN programs1 described in this report aid in the assembly of up to 100 individual overlapping DNA sequences generated by M-13 subcloning and sequencing methods. The program produces a printout of the aligned sequences presented in register. Use of the program will be facilitated because 1) it is written with the Microsoft BASIC interpreter, 2) sequence data may be entered and edited using WORDSTAR or similar word processing programs, and 3) hardware requirements for execution of the program on CP/M or MS-DOS (IBM-PC compatible) systems are minimal.
摘要
本报告中描述的SEQALIGN程序有助于组装通过M-13亚克隆和测序方法生成的多达100个单独的重叠DNA序列。该程序会输出对齐后的序列打印件，序列按对齐状态呈现。该程序的使用将得到便利，原因如下：1）它是用微软BASIC解释器编写的；2）序列数据可以使用WORDSTAR或类似的文字处理程序进行输入和编辑；3）在CP/M或MS-DOS（与IBM-PC兼容）系统上运行该程序的硬件要求极低。
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