Numazaki K, Chiba S, Kogawa K, Umetsu M, Motoya H, Nakao T
J Clin Pathol. 1986 Jan;39(1):84-8. doi: 10.1136/jcp.39.1.84.
The relation between chronic respiratory disease and infection with Chlamydia trachomatis in premature infants was investigated to ascertain the aetiological importance of intrauterine C trachomatis infection and chronic respiratory disease in premature infants. Serum IgM antibodies against C trachomatis were determined by enzyme linked fluorescence assay. Sections of lung tissues obtained by biopsy and at necropsy were also tested for the presence of antigens using fluorescein conjugated monoclonal antibodies to C trachomatis. Of 16 sera from premature infants with chronic respiratory diseases clinically diagnosed as bronchopulmonary dysplasia or the Wilson-Mikity syndrome, five had IgM antibodies to C trachomatis L2 strain by enzyme linked fluorescence assay (titre greater than or equal to 1/500). Of 37 sera from premature infants with extremely low birth weights, two had IgM antibodies to C trachomatis. No specific IgM antibody was detected in 31 neonates who showed raised serum IgM concentrations but who did not have respiratory tract symptoms. C trachomatis was identified from two specimens of lung tissue obtained at necropsy from premature infants with chronic respiratory disease positive for IgM antibody. These findings indicate the aetiological importance of intrauterine C trachomatis infection in chronic respiratory disease in premature infants.
为确定宫内沙眼衣原体感染与早产儿慢性呼吸道疾病的病因学重要性，对早产儿慢性呼吸道疾病与沙眼衣原体感染之间的关系进行了调查。采用酶联荧光分析法测定血清抗沙眼衣原体IgM抗体。还使用荧光素结合的抗沙眼衣原体单克隆抗体对通过活检和尸检获得的肺组织切片进行抗原检测。在临床上诊断为支气管肺发育不良或威尔逊 - 米基蒂综合征的患有慢性呼吸道疾病的早产儿的16份血清中，通过酶联荧光分析法，有5份对沙眼衣原体L2菌株具有IgM抗体（滴度大于或等于1/500）。在出生体重极低的早产儿的37份血清中，有2份对沙眼衣原体具有IgM抗体。在31例血清IgM浓度升高但无呼吸道症状的新生儿中未检测到特异性IgM抗体。从尸检获得的2份患有慢性呼吸道疾病且IgM抗体呈阳性的早产儿肺组织标本中鉴定出沙眼衣原体。这些发现表明宫内沙眼衣原体感染在早产儿慢性呼吸道疾病中的病因学重要性。
