Biomaterials and Multiscale Mechanics Lab, Department of Metallurgical and Materials Engineering, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand 247667, India; Molecular Endocrinology Lab, Department of Biosciences and Bioengineering, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand 247667, India; Centre for Nanotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand 247667, India.
Biomaterials and Multiscale Mechanics Lab, Department of Metallurgical and Materials Engineering, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand 247667, India; Centre for Nanotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand 247667, India.
Biomater Adv. 2023 Nov;154:213596. doi: 10.1016/j.bioadv.2023.213596. Epub 2023 Aug 25.
Degenerative central nervous system (CNS) disorders and traumatic brain injuries are common nowadays. These may induce the loss of neuronal cells and delicate connections essential for optimal CNS function. The CNS tissue has restricted regeneration ability, hindering the development of effective therapies. Developing cell and tissue instructive materials may bring up new treatment possibilities. In this study, chitosan-graphene nano platelets (GNPs) composite films were developed to regenerate brain cells. This study evaluates the effects of GNP concentration (0.5, 1 and 2 wt%) and their alignment on mechanical, electrical, surface, protein adsorption and biological properties of the regenerative scaffolds. Incorporating and aligning GNPs into chitosan matrix improved all the physical and biological properties. On reinforced scaffolds, HT22 cell morphology mimics pyramidal brain cells, which are responsible for the brain's highly branched neural network. Additionally, the reinforced scaffolds supported Mesenchymal Stem like Cells growth and were biocompatible in vivo. The alignment of GNPs in the chitosan matrix offered the appropriate physicochemical and biological properties to promote adhesion, proliferation and shape morphogenesis of hippocampal HT22 neuronal cells. Overall, this study delineates the enormous potential offered by the GNP-reinforced scaffolds for regeneration of central nervous system, especially the brain.
如今，退行性中枢神经系统（CNS）疾病和外伤性脑损伤很常见。这些疾病可能导致神经元细胞和对中枢神经系统功能至关重要的精细连接的丧失。中枢神经系统组织的再生能力有限，阻碍了有效治疗方法的发展。开发细胞和组织指导材料可能会带来新的治疗可能性。在这项研究中，壳聚糖-石墨烯纳米片（GNPs）复合膜被开发用于脑细胞再生。本研究评估了 GNP 浓度（0.5、1 和 2wt%）及其对齐方式对再生支架的机械、电气、表面、蛋白质吸附和生物学特性的影响。将 GNPs 掺入壳聚糖基质并使其对齐可改善所有物理和生物学特性。在增强的支架上，HT22 细胞形态模拟负责大脑高度分支神经网络的脑锥体神经元。此外，增强的支架支持间充质干细胞样细胞的生长，并在体内具有生物相容性。GNPs 在壳聚糖基质中的排列提供了适当的物理化学和生物学特性，以促进海马 HT22 神经元细胞的黏附、增殖和形态发生。总的来说，这项研究描绘了 GNP 增强支架在中枢神经系统，特别是大脑再生方面提供的巨大潜力。
