Schmitz Moniek G J, Aarts Jasper G M, Burroughs Laurence, Sudarsanam Phanikrishna, Kuijpers Tim J M, Riool Martijn, de Boer Leonie, Xue Xuan, Bosnacki Dragan, Zaat Sebastian A J, de Boer Jan, Alexander Morgan R, Dankers Patricia Y W
Department of Biomedical Engineering, Institute for Complex Molecular Systems (ICMS), Eindhoven University of Technology, PO Box 513, Eindhoven, 5600 MB, The Netherlands.
School of Pharmacy, University of Nottingham, University Park, Nottingham, NG7 2RD, UK.
Macromol Rapid Commun. 2025 Apr;46(8):e2300638. doi: 10.1002/marc.202300638. Epub 2024 Apr 13.
A polymer microarray based on the supramolecular ureido-pyrimidinone (UPy) moiety is fabricated to screen antimicrobial materials for their ability to support cell adhesion. UPy-functionalized additives, either cell-adhesive, antimicrobial or control peptides, are used, and investigated in different combinations at different concentrations, resulting in a library of 194 spots. These are characterized on composition and morphology to evaluate the microarray fabrication. Normal human dermal fibroblasts are cultured on the microarrays and cell adhesion to the spots is systematically analyzed. Results demonstrate enhanced cell adhesion on spots with combinations including the antimicrobial peptides. This study clearly proves the power of the high throughput approach in combination with supramolecular molecules, to screen additive libraries for desired biological response.
制备了一种基于超分子脲基嘧啶酮（UPy）部分的聚合物微阵列，用于筛选抗菌材料支持细胞黏附的能力。使用了UPy功能化添加剂，即细胞黏附肽、抗菌肽或对照肽，并以不同浓度进行不同组合研究，得到了一个包含194个点的文库。对这些点的组成和形态进行表征，以评估微阵列的制备情况。将正常人皮肤成纤维细胞培养在微阵列上，并系统分析细胞与这些点的黏附情况。结果表明，在包含抗菌肽组合的点上细胞黏附增强。本研究清楚地证明了高通量方法与超分子分子相结合在筛选添加剂文库以获得所需生物学反应方面的强大作用。
