通过酶联免疫吸附测定抑制技术检测念珠菌病患者血清中的念珠菌抗原。
Detection of Candida antigen in sera of patients with candidiasis by an enzyme-linked immunosorbent assay-inhibition technique.
作者信息
Segal E, Berg R A, Pizzo P A, Bennett J E
出版信息
J Clin Microbiol. 1979 Jul;10(1):116-8. doi: 10.1128/jcm.10.1.116-118.1979.
Abstract
A total of 37 serum samples from 27 cancer patients were tested by an enzyme-linked immunosorbent assay-inhibition technique for the detection of Candida antigen. In 20 randomly chosen sera from patients without clinical evidence of candidiasis and in 10 sera from patients proven by autopsy not to have candidiasis, the inhibition ranged up to 17%; in contrast, inhibition ranged from 22 to 56% in all seven patients proven by autopsy to have systemic candidiasis, indicating the presence of Candida antigen in the sera of these patients. This technique appears promising in diagnosing disseminated candidiasis in cancer patients.
摘要
采用酶联免疫吸附抑制技术对27例癌症患者的37份血清样本进行检测，以检测念珠菌抗原。在20份随机选取的无念珠菌病临床证据患者的血清以及10份经尸检证实无念珠菌病患者的血清中，抑制率高达17%；相比之下，经尸检证实患有系统性念珠菌病的所有7例患者的抑制率为22%至56%，表明这些患者的血清中存在念珠菌抗原。该技术在诊断癌症患者的播散性念珠菌病方面似乎很有前景。
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