Rosenbluh A, Mevarech M, Koltin Y, Gorman J A
Mol Gen Genet. 1985;200(3):500-2. doi: 10.1007/BF00425739.
A genomic library of the asexual pathogenic yeast Candida albicans was constructed in the S. cerevisiae vector YEp13. The library contains a representation of the entire genome with a probability of 99%. The expression of the genes of C. albicans in S. cerevisiae was examined and two mutations his3-1 and trp1-289 of S. cerevisiae were complemented by the cloned genes of C. albicans. The hybridization data indicates that the plasmids complementing the mutations of S. cerevisiae contain sequences from C. albicans.
在酿酒酵母载体YEp13中构建了无性致病酵母白色念珠菌的基因组文库。该文库包含整个基因组，其包含概率为99%。检测了白色念珠菌基因在酿酒酵母中的表达，酿酒酵母的两个突变his3-1和trp1-289被白色念珠菌的克隆基因所互补。杂交数据表明，互补酿酒酵母突变的质粒含有来自白色念珠菌的序列。
