Mio Kento, Goto Yuka, Matsuoka Tsubasa, Komatsu Mitsuko, Ishii Chiharu, Yang Jiayue, Kobayashi Toshiki, Aoe Seiichiro, Fukuda Shinji
Research and Development Department, Hakubaku co., Ltd., Yamanashi, Japan.
Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata, Japan.
NPJ Sci Food. 2024 Sep 30;8(1):69. doi: 10.1038/s41538-024-00311-9.
Barley is rich in β-glucan, which can alter gut microbiota and metabolome profiles, potentially affecting host metabolism. However, the microbiota and metabolites increased by barley β-glucan remain unclear. In this study, we focused on the gut-microbiota-derived metabolite succinate and investigated the microbiome and metabolome profiles altered by barley β-glucan intake. C57BL/6 J mice were fed a standard or middle-fat diet containing barley flour rich in β-glucan or barley flour without β-glucan, and their gut microbiota and metabolome profiles were analyzed. The results showed increased Bacteroides, Parasutterella, and succinate due to barley β-glucan intake independent of diet differences. Next, we used mice lacking slc13a2, a gene that is involved in the cellular uptake of succinate. Wild-type mice showed improved glucose tolerance after the intake of barley β-glucan, but this effect was attenuated in the slc13a2-deficient mice. These results suggest that barley β-glucan intake increases succinate and succinate-producing bacteria and affects glucose metabolism.
大麦富含β-葡聚糖，它可以改变肠道微生物群和代谢组谱，可能影响宿主代谢。然而，大麦β-葡聚糖增加的微生物群和代谢物仍不清楚。在本研究中，我们聚焦于肠道微生物群衍生的代谢物琥珀酸，并研究了大麦β-葡聚糖摄入后改变的微生物组和代谢组谱。给C57BL/6 J小鼠喂食含有富含β-葡聚糖的大麦粉或不含β-葡聚糖的大麦粉的标准或中等脂肪饮食，并分析它们的肠道微生物群和代谢组谱。结果显示，无论饮食差异如何，大麦β-葡聚糖摄入都会导致拟杆菌属、副萨特氏菌属增加以及琥珀酸增加。接下来，我们使用缺乏slc13a2基因的小鼠，该基因参与琥珀酸的细胞摄取。野生型小鼠在摄入大麦β-葡聚糖后葡萄糖耐量得到改善，但这种效应在slc13a2基因缺陷小鼠中减弱。这些结果表明，摄入大麦β-葡聚糖会增加琥珀酸和产生琥珀酸的细菌，并影响葡萄糖代谢。
