Shobha K, Ghodasara S N, Barad D B, Javia B B, Poshiya P J, Parasana D K
MVSc in Veterinary Science, Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Junagadh, India.
Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Junagadh, India.
Iran J Vet Res. 2024;25(2):117-124. doi: 10.22099/IJVR.2024.49531.7283.
Dermatological infections in dogs are challenging to treat due to antibiotic resistance, which leads to longer recovery time and the need for stronger antibiotics. This study was undertaken to determine the prevalence of antimicrobial resistance in coagulase-positive staphylococcal isolates from pyoderma infection in dogs. This study also aimed to identify isolates with methicillin-resistance and multidrug resistance.
73 coagulase-positive staphylococci isolated from varying degrees of canine pyoderma cases. The samples were analyzed for the presence of spp. using polymerase chain reaction (PCR) and resistance against antibiotics was studied by antimicrobial profile, minimum inhibitory concentration (MIC), and PCR on isolated bacteria.
Out of 75 bacterial isolates identified, 73 isolates were confirmed as species by PCR. A higher percentage of antibiotic resistance was observed against penicillin-G (46.27%), followed by amoxiclav (38.81%), enrofloxacin (32.84%), cefpodoxime, oxytetracycline (28.36% each), levofloxacin (26.86%), and co-trimoxazole (22.39%). 29 (49.15%) S. pseudintermedius three (50.00%) S. schleiferi subsp. coagulans and two (100%) S. aureus isolates exhibited multidrug resistance. However, one (1.49%) isolate (S. pseudintermedius) revealed low-level mupirocin resistance in the E-test. Also, 12 (20.34%) methicillin-resistant Staphylococcus pseudintermedius (MRSP), one (16.67%) methicillin-resistant S. schleiferi subsp. coagulans (MRSS) and one (50%) methicillin-resistant S. aureus (MRSA) were reported using PCR.
This study helps to understand the increased level and pattern of resistance in coagulase-positive staphylococci isolated from different types of canine pyoderma cases.
由于抗生素耐药性，犬类皮肤病感染的治疗具有挑战性，这导致恢复时间延长且需要更强效的抗生素。本研究旨在确定犬脓皮病感染中凝固酶阳性葡萄球菌分离株的抗菌药物耐药性流行情况。本研究还旨在鉴定耐甲氧西林和多重耐药的分离株。
从不同程度的犬脓皮病病例中分离出73株凝固酶阳性葡萄球菌。使用聚合酶链反应（PCR）分析样本中葡萄球菌属的存在情况，并通过抗菌谱、最低抑菌浓度（MIC）以及对分离细菌进行PCR来研究对抗生素的耐药性。
在鉴定出的75株细菌分离株中，73株通过PCR确认为葡萄球菌属。观察到对青霉素G的耐药率较高（46.27%），其次是阿莫西林克拉维酸（38.81%）、恩诺沙星（32.84%）、头孢泊肟、土霉素（均为28.36%）、左氧氟沙星（26.86%）和复方新诺明（22.39%）。29株（49.15%）中间葡萄球菌、3株（50.00%）施氏葡萄球菌凝固酶亚种和2株（100%）金黄色葡萄球菌分离株表现出多重耐药性。然而，1株（1.49%）分离株（中间葡萄球菌）在E试验中显示出低水平的莫匹罗星耐药性。此外，使用PCR报告了12株（20.34%）耐甲氧西林中间葡萄球菌（MRSP）、1株（16.67%）耐甲氧西林施氏葡萄球菌凝固酶亚种（MRSS）和1株（50%）耐甲氧西林金黄色葡萄球菌（MRSA）。
本研究有助于了解从不同类型犬脓皮病病例中分离出的凝固酶阳性葡萄球菌的耐药水平和模式增加情况。
