异分支酸合酶是水稻中维生素K1合成所必需的，但不是水杨酸生物合成所必需的。
Isochorismate synthase is required for phylloquinone, but not salicylic acid biosynthesis in rice.
作者信息
Wang Zengqian, Yang Guiqing, Zhang Dandan, Li Guangxin, Qiu Jin-Long, Wu Jie
机构信息
College of Agriculture, Shanxi Agricultural University, Jinzhong, 030801 China.
State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101 China.
出版信息
aBIOTECH. 2024 May 24;5(4):488-496. doi: 10.1007/s42994-024-00166-4. eCollection 2024 Dec.
UNLABELLED
Salicylic acid (SA) is a phytohormone required for plant growth and defense signaling. There are two major SA biosynthesis pathways in plants: the isochorismate synthase (ICS) pathway and the phenylalanine ammonia-lyase (PAL) pathway. It has been demonstrated in several plant species, including the model plant , that SA is derived predominantly from the ICS pathway. Here, we employed the CRISPR/Cas9 system to generate knockout mutants in rice ( L.). The mutants display severe growth defects, and are completely devoid of phylloquinone, an isochorismate-derived product. The growth defects of can be rescued through exogenous application of 1,4-dihydroxy-2-naphthoic acid (NA), a precursor of phylloquinone. Remarkably, the basal SA levels are not altered in the mutants. Our findings support a role of OsICS in the biosynthesis of phylloquinone, and imply that SA biosynthesis in rice may occur through an alternative route other than the ICS pathway.
SUPPLEMENTARY INFORMATION
The online version contains supplementary material available at 10.1007/s42994-024-00166-4.
未标记
水杨酸（SA）是植物生长和防御信号传导所需的一种植物激素。植物中有两条主要的SA生物合成途径：异分支酸合酶（ICS）途径和苯丙氨酸解氨酶（PAL）途径。在包括模式植物在内的几种植物物种中已证明，SA主要来源于ICS途径。在此，我们利用CRISPR/Cas9系统在水稻（）中产生敲除突变体。突变体表现出严重的生长缺陷，并且完全缺乏维生素K1（一种异分支酸衍生产物）。突变体的生长缺陷可以通过外源施加维生素K1的前体1,4 - 二羟基 - 2 - 萘甲酸（NA）来挽救。值得注意的是，突变体中的基础SA水平没有改变。我们的研究结果支持了水稻OsICS在维生素K1生物合成中的作用，并暗示水稻中SA的生物合成可能通过ICS途径以外的替代途径发生。
补充信息
在线版本包含可在10.1007/s42994 - 024 - 00166 - 4获取的补充材料。
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