Jiang Shuo, Ye Mengmin, Liu Ke, Cao Huiluo, Lin Xiaoshan
Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China.
Department of Laboratory Medicine, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China.
BMC Genom Data. 2025 Jan 22;26(1):7. doi: 10.1186/s12863-025-01298-2.
Pseudomonas aeruginosa, a Gram-negative opportunistic pathogen, is frequently associated with multidrug resistance and global epidemic outbreaks, contributing significantly to morbidity and mortality in hospitalized patients. However, P. aeruginosa belonging to the sequence type (ST) 16 was rarely reported. Here, this report presents the complete genome sequence of YK01, a ST16 P. aeruginosa isolate from a patient with keratitis. The complete reference genome of P. aeruginosa YK01 is expected to provide valuable data for investigating its genomic population, enhancing understanding of genetic basis of P. aeruginosa species complex.
A complete genome of 6.3 Mb was obtained for P. aeruginosa YK01 by combining Illumina 150-bp short reads and Nanopore long reads. The assembly is fully complete with chromosomal genome size of 6,183,266 bp, presenting a GC content of 66.7%, and a plasmid with the size of 46,067 bp, presenting GC content of 59.0%. Predicted chromosomal genomic features include 5,709 CDS, 12 rRNAs, 63 tRNAs, 4 ncRNAs, and 5,788 genes. To our knowledge, this genome data represents the first complete genome of P. aeruginosa ST16, providing crucial information for further comparative genome analysis.
铜绿假单胞菌是一种革兰氏阴性机会致病菌，常与多重耐药和全球流行暴发相关，对住院患者的发病率和死亡率有重大影响。然而，属于序列型（ST）16的铜绿假单胞菌报道较少。在此，本报告展示了一株来自角膜炎患者的ST16铜绿假单胞菌YK01的完整基因组序列。铜绿假单胞菌YK01的完整参考基因组有望为研究其基因组群体提供有价值的数据，增进对铜绿假单胞菌种复合体遗传基础的理解。
通过结合Illumina 150 bp短读长和Nanopore长读长，获得了铜绿假单胞菌YK01的一个6.3 Mb的完整基因组。组装完全完成，染色体基因组大小为6,183,266 bp，GC含量为66.7%，还有一个大小为46,067 bp的质粒，GC含量为59.0%。预测的染色体基因组特征包括5,709个编码序列、12个核糖体RNA、63个转运RNA、4个非编码RNA和5,788个基因。据我们所知，该基因组数据代表了铜绿假单胞菌ST16的首个完整基因组，为进一步的比较基因组分析提供了关键信息。
