Ribes G, Blayac J P, Valette G, Loubatières-Mariani M M
J Physiol (Paris). 1979;75(8):881-6.
Various types of experimental hyperlactatemia were induced in the normal anesthetized dog, and the changes in insulin secretion were measured in the pancreatico-duodenal vein. Hyperlactatemia was induced in the absence or in the presence of an infusion of sodium dichloroacetate (DCA), which activates pyruvate dehydrogenase. 1. Exogenous hyperlactatemia: The infusion of sodium L(+)lactate resulted in a strong increase in blood lactate level which was accompanied by a significant increase in the insulin output from the pancreatico-duodenal vein. The administration of DCA did not counteract the increase in lactate level and did not modify insulin output either. 2. Endogenous hyperlactatemia: This was induced either by pharmacological means: the subcutaneous injection of an antidiabetic biguanide, phenformin (20 mg/kg), or by physiological means: intense muscular work. In both cases an increase in the lactate level and in insulin output was recorded. The administration of DCA suppressed the hyperlactatemia and counteracted the increase in insulin output. These results show that there is a relationship between lactate level and insulin secretion, and give evidence for a role of endogenous lactate in the regulation of insulin secretion.
在正常麻醉犬身上诱导出各种类型的实验性高乳酸血症，并在胰十二指肠静脉中测量胰岛素分泌的变化。在不存在或存在激活丙酮酸脱氢酶的二氯乙酸钠（DCA）输注的情况下诱导高乳酸血症。1. 外源性高乳酸血症：输注L(+)乳酸钠导致血乳酸水平大幅升高，同时胰十二指肠静脉的胰岛素输出量显著增加。给予DCA既不能抵消乳酸水平的升高，也不能改变胰岛素输出量。2. 内源性高乳酸血症：这要么通过药理学方法诱导：皮下注射抗糖尿病双胍类药物苯乙双胍（20mg/kg），要么通过生理学方法诱导：剧烈肌肉运动。在这两种情况下，均记录到乳酸水平和胰岛素输出量增加。给予DCA可抑制高乳酸血症并抵消胰岛素输出量的增加。这些结果表明乳酸水平与胰岛素分泌之间存在关联，并证明内源性乳酸在胰岛素分泌调节中起作用。
