关于双扩散试验在阴道念珠菌病诊断中的应用观察
Observations on the use of the double diffusion test in the diagnosis of vaginal candidiasis.
作者信息
Jones G R, Warnock D W
出版信息
J Clin Pathol. 1977 Mar;30(3):262-5. doi: 10.1136/jcp.30.3.262.
Precipitins to mannan and cytoplasmic antigens of three Candida species were determined in an unselected series of 289 non-pregnant women. Precipitins were present in 20% of sera of women with vaginal candidiasis, in 23% of women harbouring yeasts in the vagina without clinical signs of infection, and in 21% of women harbouring yeasts in sites other than the vagina. Of the 47 patients who reacted with Candida albicans mannan or cytoplasmic antigens, 98% reacted with mannan antigen but only 13% with cytoplasmic antigen. The inclusion of mannan and cytoplasmic antigens of C. guilliermondii and C. parapsilosis did not increase the specificity or sensitivity of the test in the diagnosis of vaginal candidiasis. It is suggested that the double diffusion test is of doubtful value as an adjunct to the diagnosis of vaginal candidiasis.
在289名未经挑选的非妊娠女性中测定了针对三种念珠菌属甘露聚糖和细胞质抗原的沉淀素。患有阴道念珠菌病的女性血清中，20%存在沉淀素；阴道中有酵母菌但无感染临床体征的女性中，23%存在沉淀素；阴道以外部位有酵母菌的女性中，21%存在沉淀素。在47名与白色念珠菌甘露聚糖或细胞质抗原发生反应的患者中，98%与甘露聚糖抗原发生反应，而与细胞质抗原发生反应的仅占13%。添加季也蒙念珠菌和近平滑念珠菌的甘露聚糖和细胞质抗原，并未提高该试验在诊断阴道念珠菌病中的特异性或敏感性。提示双扩散试验作为阴道念珠菌病诊断的辅助手段，价值存疑。
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