Wehr C T, Kudrna R D, Parks L W
J Bacteriol. 1970 Jun;102(3):636-41. doi: 10.1128/jb.102.3.636-641.1970.
The effects of inhibitors of bacterial deoxyribonucleic acid (DNA) synthesis upon logarithmically growing cultures of Saccharomyces cerevisiae were investigated. Cell division, ribonucleic acid (RNA) synthesis, and DNA synthesis were measured after addition of nalidixic acid, fluorodeoxyuridine, or phenethyl alcohol to cultures of yeast growing in defined and complex media. Both nalidixic acid and fluorodeoxyuridine had only temporary effects on nucleic acid synthesis in cultures growing in defined medium, and little or no observable effect on cultures growing in complex medium. Neither compound inhibited colony formation on complex solid medium, although growth was slow on defined solid medium. Phenethyl alcohol caused complete inhibition of DNA synthesis, RNA synthesis, and cell division in cultures growing in defined medium. In cultures growing in complex medium, RNA synthesis and cell division were inhibited to a lesser extent. A slight increase in DNA was observed in the presence of the inhibitor.
研究了细菌脱氧核糖核酸（DNA）合成抑制剂对对数生长期酿酒酵母培养物的影响。在向生长于限定培养基和复合培养基中的酵母培养物中添加萘啶酸、氟脱氧尿苷或苯乙醇后，测量细胞分裂、核糖核酸（RNA）合成和DNA合成。萘啶酸和氟脱氧尿苷对生长于限定培养基中的培养物的核酸合成仅产生暂时影响，对生长于复合培养基中的培养物几乎没有或没有可观察到的影响。这两种化合物均未抑制复合固体培养基上的菌落形成，尽管在限定固体培养基上生长缓慢。苯乙醇导致生长于限定培养基中的培养物的DNA合成、RNA合成和细胞分裂完全受到抑制。在生长于复合培养基中的培养物中，RNA合成和细胞分裂受到的抑制程度较小。在抑制剂存在的情况下，观察到DNA略有增加。
