Lilly H A, Lowbury E J, Wilkins M D, Zaggy A
J Hyg (Lond). 1979 Jun;82(3):497-500. doi: 10.1017/s0022172400054024.
Aqueous suspensions of Staphylococcus aureus were deposited on a Millipore filter and then exposed for a few seconds to 70% ethyl alcohol. Viable counts of bacteria extracted from the filter immediately after exposure to alcohol, and, in replicate experiments, after a further period of 3 h, showed that the mean immediate reduction of 97.6% in viable counts after treatment with alcohol was followed by a further mean reduction of 67.1% in the further 3 h holding time; the same bacterial suspensions allowed to dry on Millipore filters without exposure to alcohol showed a significantly smaller mean reduction in viable counts (34.3%) during a further 3 h holding time. These findings support the view that the reported further fall in numbers of bacteria on hands while wearing gloves for 3 h after alcohol disinfection can be explained by sublethal damage to some of the bacteria, from which they can recover only if promptly inoculated on culture medium.
将金黄色葡萄球菌的水悬浮液沉积在微孔滤膜上，然后暴露于70%乙醇中几秒钟。在暴露于酒精后立即从滤膜中提取的细菌活菌计数，以及在重复实验中，在另外3小时后提取的细菌活菌计数表明，用酒精处理后活菌计数平均立即减少97.6%，随后在再延长的3小时保持时间内平均又减少67.1%；同样的细菌悬浮液在微孔滤膜上干燥而不暴露于酒精，在再延长的3小时保持时间内活菌计数的平均减少幅度明显较小（34.3%）。这些发现支持了这样一种观点，即报告称酒精消毒后戴手套3小时内手上细菌数量进一步下降，这可以用一些细菌受到亚致死损伤来解释，只有将它们迅速接种在培养基上，它们才能从中恢复。
