Siegel B V, Brown M, Morton J I
Immunology. 1972 Mar;22(3):457-63.
Employing formalinized chicken erythrocyte nuclei as substrate for indirect immunofluorescence assay it was possible to detect antinuclear antibodies (ANA) in the plasma of very young New Zealand Black (NZB) mice. While 7–10-day-old animals gave uniformly negative responses, weak ANA was detected in about 12 per cent of NZB mice at 14 days of age, with strong reactivity demonstrable in 90 per cent of mice by the time they were 5 weeks old. Quantification of immunofluorescence indicated that ANA reactivity of NZB plasma increased with age as measured up to 2 years. During the first year of life, mice of C57B1, CBA, C3H, BALB/c, AKR, A, SJL and 129/J strains manifested largely negative and weak reactions. However, by 1–2 years of age these animals showed marked increases in incidence of strongly reactive ANA.
以甲醛固定的鸡红细胞核为间接免疫荧光测定的底物，有可能在非常年幼的新西兰黑（NZB）小鼠血浆中检测到抗核抗体（ANA）。7至10日龄的动物反应均为阴性，14日龄的NZB小鼠中约12%检测到弱阳性ANA，到5周龄时，90%的小鼠表现出强反应性。免疫荧光定量表明，NZB血浆的ANA反应性随年龄增长而增加，最长可测量到2年。在出生后的第一年，C57B1、CBA、C3H、BALB/c、AKR、A、SJL和129/J品系的小鼠大多表现为阴性和弱阳性反应。然而，到1至2岁时，这些动物中强反应性ANA的发生率显著增加。
