Mel D M, Terzin A L, Vuksić L
Bull World Health Organ. 1965;32(5):633-6.
In the course of a series of experiments begun in 1954 a number of different killed vaccines prepared from Shigella flexneri 1 and 2a serotypes were tested for their immunogenicity after intraperitoneal administration in mice followed by challenge with virulent organisms of the same serotype; some live vaccines were also tested intraperitoneally or orally.Maintenance of the mice on Freter's regimen proved a reliable method of rendering them susceptible to intestinal infection with streptomycin-resistant shigellae.Two killed vaccines conferred good protection against intracerebral challenge, but neither live nor killed vaccines administered intraperitoneally protected against oral infection of mice maintained on Freter's regimen. However, oral administration of live vaccine did confer significant protection against homologous oral challenge, the degree of protection depending on the dose of vaccine.
在始于1954年的一系列实验过程中，对多种由福氏志贺菌1型和2a血清型制备的不同灭活疫苗进行了测试，方法是在小鼠腹腔注射后，用相同血清型的强毒菌株进行攻击，以检测其免疫原性；还对一些活疫苗进行了腹腔内或口服测试。事实证明，按照弗雷特尔方案饲养小鼠是使它们易受耐链霉素志贺菌肠道感染的可靠方法。两种灭活疫苗对脑内攻击提供了良好的保护，但无论是腹腔注射活疫苗还是灭活疫苗，都不能保护按照弗雷特尔方案饲养的小鼠免受口服感染。然而，口服活疫苗确实对同源口服攻击提供了显著的保护，保护程度取决于疫苗剂量。
