Thomassen M J, Klinger J D, Winnie G B, Wood R E, Burtner C, Tomashefski J F, Horowitz J G, Tandler B
Infect Immun. 1984 Sep;45(3):741-7. doi: 10.1128/iai.45.3.741-747.1984.
A model of chronic pulmonary infection was used for studying cellular events in a sequential manner. In this model, agarose beads containing Pseudomonas aeruginosa were instilled endotracheally into cats. Nine cats were inoculated with agarose beads containing P. aeruginosa, and four others were inoculated with sterile beads. With a fiberoptic bronchoscope, bronchial washings were obtained biweekly for up to 30 weeks. The quantitative pulmonary inflammatory cell response and alveolar macrophage morphology of the animals exposed to P. aeruginosa were compared with those for the animals exposed to a chronic irritant (agarose beads). Bronchial washings of all animals before inoculation showed that 70 to 90% of the cells were macrophages. After inoculation with P. aeruginosa, a persistent inflammatory response was observed (60 to 70% granulocytes). In the sterile-bead-inoculated group, the response was less prominent (30 to 40% granulocytes). As early as 2 weeks after inoculation, alveolar macrophages from infected animals were larger and had cytoplasmic features that differed from those of controls. Electron microscope examination showed prominent surface alterations in alveolar macrophages from the infected cats. These alterations persisted from 2 to 12 weeks after infection. In animals inoculated with sterile beads, alveolar macrophages exhibited less extensive surface changes that had resolved by week 8. Histologically, chronic bronchiolitis and pneumonia were more severe in the infected animals than in controls. This model of chronic inflammation and macrophage stimulation, which is similar to the chronic pneumonia of cystic fibrosis, may be a useful approach to answer questions on the role of macrophage activation in chronic lung disease.
采用慢性肺部感染模型对细胞事件进行序贯研究。在该模型中，将含有铜绿假单胞菌的琼脂糖珠经气管内注入猫体内。9只猫接种含铜绿假单胞菌的琼脂糖珠，另外4只接种无菌珠。使用纤维支气管镜，每两周获取支气管灌洗液，最长持续30周。将暴露于铜绿假单胞菌的动物的定量肺部炎症细胞反应和肺泡巨噬细胞形态与暴露于慢性刺激物（琼脂糖珠）的动物进行比较。所有动物接种前的支气管灌洗液显示，70%至90%的细胞为巨噬细胞。接种铜绿假单胞菌后，观察到持续的炎症反应（60%至70%为粒细胞）。在接种无菌珠的组中，反应不那么明显（30%至40%为粒细胞）。接种后早在2周，感染动物的肺泡巨噬细胞就更大，且具有与对照不同的细胞质特征。电子显微镜检查显示感染猫的肺泡巨噬细胞表面有明显改变。这些改变在感染后2至12周持续存在。在接种无菌珠的动物中，肺泡巨噬细胞表面变化范围较小，在第8周时已消退。组织学上，感染动物的慢性细支气管炎和肺炎比对照更严重。这种慢性炎症和巨噬细胞刺激模型类似于囊性纤维化的慢性肺炎，可能是回答巨噬细胞活化在慢性肺病中作用问题的一种有用方法。
