Lehmann P F, Reiss E
Mycopathologia. 1980 Mar 17;70(2):83-8. doi: 10.1007/BF00443072.
Anti-mannan was produced in rabbits after peptidoglucomannan in adjuvant was injected. The antiserum was used to detect mannan by immunodiffusion and counterimmunoelectrophoresis (CIE) in gel and by sandwich enzyme-linked immunosorbent assay (ELISA). The antiserum detected lower concentrations of mannan of serotype A than of serotype B. Except in CIE, the reactions were more pronounced at 4 degrees C than at higher temperatures. CIE detected 0.8 microgram/ml mannan A or 12.5 microgram/ml mannan B. Sandwich ELISA detected 3 ng/ml mannan A or 10(5) ng/ml mannan B. Mannan was not detected in the serum of patients or rabbits with candidiasis.
在兔体内注射佐剂中的肽葡甘露聚糖后产生了抗甘露聚糖。用该抗血清通过凝胶免疫扩散和对流免疫电泳（CIE）以及夹心酶联免疫吸附测定（ELISA）检测甘露聚糖。该抗血清检测到的A型血清型甘露聚糖浓度低于B型血清型。除了在CIE中，4℃时的反应比在较高温度时更明显。CIE检测到0.8微克/毫升的A型甘露聚糖或12.5微克/毫升的B型甘露聚糖。夹心ELISA检测到3纳克/毫升的A型甘露聚糖或10⁵纳克/毫升的B型甘露聚糖。在念珠菌病患者或兔的血清中未检测到甘露聚糖。
