Ishida K, Guze P A, Kalmanson G M, Albrandt K, Guze L B
Antimicrob Agents Chemother. 1982 Apr;21(4):688-90. doi: 10.1128/AAC.21.4.688.
Certain technical considerations which affected the status of methicillin tolerance in Staphylococcus aureus strains were studied. Methods which consistently demonstrated tolerance or intolerance of a given strain were avoidance of inoculum splashing, use of stationary-phase inoculum, 24-h tube incubation, and minimization of antibiotic carry-over. These studies suggested a need for the establishment of a standardized reference for the determination of tolerance.
研究了影响金黄色葡萄球菌菌株对甲氧西林耐受性状况的某些技术因素。能够始终证明某一特定菌株耐受性或不耐受性的方法包括避免接种物飞溅、使用稳定期接种物、24小时试管培养以及尽量减少抗生素残留。这些研究表明需要建立一个用于确定耐受性的标准化参考标准。