Kang D, Rothman N, Cho S H, Lim H S, Kwon H J, Kim S M, Schwartz B, Strickland P T
Department of Environmental Health Sciences, Johns Hopkins School of Public Health, Baltimore, MD 21205, USA.
Occup Environ Med. 1995 Sep;52(9):593-9. doi: 10.1136/oem.52.9.593.
Increased risk of lung cancer has been associated with employment in the steel industry. This association is thought to be due in part to increased concentrations of polycyclic aromatic hydrocarbons (PAHs) in air found in this work environment. Measurement of PAH metabolites in human urine provides a means of assessing individual internal dose of PAHs. This study examined the relative contribution of occupation and smoking to urinary concentration of 1-hydroxypyrene glucuronide (1-OHPG) among a group of workers at a steel plant.
Concentrations of 1-OHPG in urine from 44 workers with jobs associated with increased air concentrations of PAHs and 40 workers with jobs with low or no exposure to PAHs were measured. 20 workers in each group were not current smokers. Urinary 1-OHPG was measured by synchronous fluorescence spectroscopy after immunoaffinity chromatography specific for PAH metabolites.
Mean (SEM) urinary 1-OHPG concentration was 2.16 (0.42) pmol/ml urine among the 44 occupationally exposed workers compared with 0.38 (0.05) among the 40 workers with no or low exposure (P < 0.0001). Mean urinary 1-OHPG concentration was 1.82 (0.41) pmol/ml urine among the 44 current smokers compared with 0.75 (0.20) among the 40 non-smokers (P < 0.005). Mean 1-OHPG concentrations in non-smokers were 0.26 (n = 20), 0.70 (n = 15), and 2.84 pmol/ml urine (n = 5) for strata of exposure to PAHs (no or low, mid, and high) based on job category; the corresponding values in smokers were 0.55 (n = 20), 0.94 (n = 12), and 4.91 pmol/ml (n = 12), respectively. Multiple linear regression showed significant differences between subjects in different PAH exposure with increased concentrations of 1-OHPG in urine. Amounts of foods containing PAHs ingested by this group of workers were relatively low and did not contribute significantly to urinary 1-OHPG concentrations.
These results indicate that 1-OHPG is a common urinary metabolite in people with recent occupational exposure to PAHs and is associated with both job category and estimated stratum of PAH exposure.
肺癌风险增加与钢铁行业就业有关。这种关联被认为部分归因于该工作环境空气中多环芳烃（PAHs）浓度的增加。测量人体尿液中的PAH代谢物可提供一种评估个体PAHs内部剂量的方法。本研究调查了一家钢铁厂一组工人中职业和吸烟对尿中1-羟基芘葡萄糖醛酸苷（1-OHPG）浓度的相对贡献。
测量了44名从事与空气中PAHs浓度增加相关工作的工人以及40名低暴露或无暴露于PAHs工作的工人尿液中1-OHPG的浓度。每组中有20名工人目前不吸烟。采用针对PAH代谢物的免疫亲和色谱法后，通过同步荧光光谱法测量尿中1-OHPG。
44名职业暴露工人尿中1-OHPG的平均（SEM）浓度为2.16（0.42）pmol/ml，而40名低暴露或无暴露工人的该浓度为0.38（0.05）pmol/ml（P < 0.0001）。44名当前吸烟者尿中1-OHPG的平均浓度为1.82（0.41）pmol/ml，而40名不吸烟者的该浓度为0.75（0.20）pmol/ml（P < 0.005）。根据工作类别，非吸烟者中PAHs暴露分层（无或低、中、高）的尿中1-OHPG平均浓度分别为0.26（n = 20）、0.70（n = 15）和2.84 pmol/ml（n = 5）；吸烟者中的相应值分别为0.55（n = 20）、0.94（n = 12）和4.91 pmol/ml（n = 12）。多元线性回归显示，不同PAH暴露的受试者之间尿中1-OHPG浓度增加存在显著差异。该组工人摄入的含PAHs食物量相对较低，对尿中1-OHPG浓度没有显著贡献。
这些结果表明，1-OHPG是近期职业暴露于PAHs人群中常见的尿代谢物，并且与工作类别和估计的PAH暴露分层均相关。
