Leonhardt A, Olsson J, Dahlén G
Department of Oral Microbiology, Faculty of Odontology, University of Göteborg, Sweden.
J Dent Res. 1995 Sep;74(9):1607-12. doi: 10.1177/00220345950740091701.
A study was conducted to evaluate qualitative and quantitative differences in bacterial colonization on titanium, hydroxyapatite, and amalgam surfaces in vivo. Six healthy adult individuals participated in the study. Two pieces each of titanium, hydroxyapatite, and amalgam of similar size were placed in cobalt-chromium splints and kept intra-orally in each individual for 10 min, and 1, 3, 6, 24, and 72 hrs. After removal of the splints, the pieces were rinsed in PBS and transferred to transport medium. After being vortexed, the samples were inoculated on selective and non-selective media for analyses of various facultative and anaerobic bacteria. During the experiment, total viable count increased on all surfaces. The investigated bacterial groups constituted, on average, approximately 60 to 99% of the total viable count on all three types of surfaces in each of the experiments, except in the 10-minute samples, when they constituted around 20 to 30%. Various streptococcal species predominated and usually constituted > 50% of total viable count. Similar colonization patterns of Streptococcus spp., Actinomyces naeslundii, Neisseria spp., Hemophilus parainfluenzae, Fusobacterium spp., and black-pigmented Prevotella spp. were seen at all three types of surfaces. No significant differences among the materials regarding colonization of investigated bacteria were found during the study period. This study failed to show any qualitative and quantitative differences in bacterial colonization among these materials. Titanium, hydroxyapatite, and amalgam do not seem to have a marked influence on the early colonization pattern in vivo.
进行了一项研究，以评估体内钛、羟基磷灰石和汞合金表面细菌定植的定性和定量差异。六名健康成年个体参与了该研究。将大小相似的钛、羟基磷灰石和汞合金各两片置于钴铬夹板中，在每个个体口腔内分别放置10分钟、1小时、3小时、6小时、24小时和72小时。取出夹板后，将样本在磷酸盐缓冲盐水中冲洗，然后转移至运输培养基中。涡旋后，将样本接种在选择性和非选择性培养基上，以分析各种兼性菌和厌氧菌。在实验过程中，所有表面的总活菌数均增加。在每个实验中，除10分钟样本中外，所研究的细菌组平均占所有三种表面总活菌数的约60%至99%，在10分钟样本中，它们占总活菌数的约20%至30%。各种链球菌属占主导地位，通常占总活菌数的>50%。在所有三种表面均观察到链球菌属、内氏放线菌、奈瑟菌属、副流感嗜血杆菌、梭杆菌属和产黑色素普雷沃菌属的相似定植模式。在研究期间，未发现材料之间在所研究细菌的定植方面存在显著差异。这项研究未能显示这些材料之间在细菌定植方面存在任何定性和定量差异。钛、羟基磷灰石和汞合金似乎对体内早期定植模式没有显著影响。
