Fukahori T, Tomioka T, Inoue K, Tajima Y, Tsunoda T, Kanematsu T
Second Department of Surgery, Nagasaki University School of Medicine, Japan.
Virchows Arch A Pathol Anat Histopathol. 1993;422(3):233-8. doi: 10.1007/BF01621807.
A subcutaneously transplantable cancer line from the intrahepatic bile duct (IHBD) induced by N-nitrosobis(2-oxopropyl) amine was established in Syrian golden hamsters. The doubling time of this tumour was 2.6 days when 2 x 10(5) tumour cells were inoculated subcutaneously (take-up rate was 100%). Growth of the tumour was significantly faster in male hamsters but neither oestrogen nor androgen receptors were detected in the tumour. The primary and all allograft tumours were tubular adenocarcinomas with fibrosis and a scirrhous pattern resembling human IHBD carcinoma of the peripheral type. Transmission electron microscopic findings showed irregular glands covered with numerous microvilli. Blood-group-related antigens including A, B and H were positive. P-Glycoprotein, which is an indicator of multidrug resistance, was also positive. Carcinoembryonic antigen and CA19-9 as general tumour markers of the biliary tract were negative. The deoxyribonucleic acid (DNA) pattern of this transplantable carcinoma was diploid. This newly established animal model of a transplantable IHBD carcinoma can be used to examine the mechanisms of synthesis and secretion of tumour-associated antigens and to study potential therapeutic agents.
通过N-亚硝基双（2-氧代丙基）胺诱导建立了叙利亚金黄地鼠肝内胆管（IHBD）皮下可移植癌系。当皮下接种2×10⁵个肿瘤细胞时（接种率为100%），该肿瘤的倍增时间为2.6天。雄性地鼠体内肿瘤生长明显更快，但肿瘤中未检测到雌激素或雄激素受体。原发性和所有同种异体移植肿瘤均为管状腺癌，伴有纤维化和硬癌模式，类似于人类外周型IHBD癌。透射电子显微镜检查结果显示，腺体不规则，表面有许多微绒毛。包括A、B和H在内的血型相关抗原呈阳性。作为多药耐药指标的P-糖蛋白也呈阳性。作为胆道一般肿瘤标志物的癌胚抗原和CA19-9均为阴性。这种可移植癌的脱氧核糖核酸（DNA）模式为二倍体。这个新建立的可移植IHBD癌动物模型可用于研究肿瘤相关抗原的合成和分泌机制以及研究潜在的治疗药物。
