Peng J, Yao X, Kobayashi H, Ma C
Department of Phytochemistry, Shenyang College of Pharmacy, People's Republic of China.
Planta Med. 1995 Feb;61(1):58-61. doi: 10.1055/s-2006-958000.
Further studies by means of preparative HPLC led to the isolation of two new furostanol saponins, macrostemonosides G (1) and I (3), along with an artifact, macrostemonoside H (2) from the bulbs of Allium macrostemon Bunge. On the basis of chemical evidence and spectral analyses (1H-,13C-NMR,1H-1H COSY,1H-13C COSY, HMBC and FAB-MS), the structure of 1 was established as 26-O-beta-D-glucopyranosyl-22-hydroxy-5 beta-furost-25(27)-ene-3 beta,12 beta,26 triol 3-O-beta-D-glucopyranosyl(1-->2)-beta-D-galactopyranoside and 2 as the 22-methoxy derivative of 1; 3 was deduced to be 26-O-beta-D-glucopyranosyl-22-hydroxy-5 beta-furost-25(27)-ene-12-one-3 beta,26- diol 3-O-beta-D-glucopyranosyl(1-->2)-beta-D-galactopyranoside. Preliminary pharmacological tests showed that macrostemonoside G (1) could inhibit ADP-induced human platelet aggregation in vitro (IC50 = 0.871 mM).
通过制备型高效液相色谱法进一步研究，从薤白鳞茎中分离出两种新的呋甾烷醇皂苷，即大薤白皂苷G（1）和I（3），以及一种人工产物大薤白皂苷H（2）。基于化学证据和光谱分析（1H-、13C-NMR、1H-1H COSY、1H-13C COSY、HMBC和FAB-MS），确定1的结构为26-O-β-D-吡喃葡萄糖基-22-羟基-5β-呋甾-25(27)-烯-3β,12β,26-三醇3-O-β-D-吡喃葡萄糖基(1→2)-β-D-吡喃半乳糖苷，2为1的22-甲氧基衍生物；推断3为26-O-β-D-吡喃葡萄糖基-22-羟基-5β-呋甾-25(27)-烯-12-酮-3β,26-二醇3-O-β-D-吡喃葡萄糖基(1→2)-β-D-吡喃半乳糖苷。初步药理试验表明，大薤白皂苷G（1）在体外可抑制ADP诱导的人血小板聚集（IC50 = 0.871 mM）。
