Lehmann A K, Halstensen A, Sørnes S, Røkke O, Waage A
Medical Department B, University of Bergen, Norway.
Infect Immun. 1995 Jun;63(6):2109-12. doi: 10.1128/iai.63.6.2109-2112.1995.
Interleukin 10 (IL-10) suppresses the production of proinflammatory cytokines in vitro and in murine models of endotoxemia and has been suggested as a candidate for treatment of bacterial septicemia. To investigate the role of IL-10 in meningococcal disease, a sandwich IL-10 enzyme-amplified sensitivity immunoassay was used to quantitate IL-10 in serum and cerebrospinal fluid samples from 41 patients with meningococcal bacteremia or meningitis with or without septic shock. High levels of IL-10 were demonstrated in sera from patients with meningococcal septic shock (mean, 21,221 pg/ml; range, 25 to 64,500 pg/ml). All cases involving fatalities had IL-10 levels in serum of > or = 1,000 pg/ml (mean, 23,058 pg/ml; range, 1,000 to 64,500 pg/ml). Patients with meningococcal meningitis without septic shock had comparably low concentrations of IL-10 in serum (mean, 119 pg/ml; range, 0 to 1,050 pg/ml) but exhibited compartmentalized release of IL-10 in cerebrospinal fluid. Concentrations of IL-10 in serum were positively correlated with the previously reported concentrations of tumor necrosis factor alpha, IL-6, and IL-8 in serum in the same patients. We conclude that IL-10 is extensively activated along with the proinflammatory cytokines during the initial phase of meningococcal septic shock and that IL-10 is associated with fatality in meningococcal disease.
白细胞介素10（IL-10）在体外以及内毒素血症小鼠模型中可抑制促炎细胞因子的产生，有人提出它可作为治疗细菌性败血症的候选药物。为了研究IL-10在脑膜炎球菌病中的作用，采用夹心式IL-10酶放大灵敏度免疫测定法对41例患有或未患有感染性休克的脑膜炎球菌血症或脑膜炎患者的血清和脑脊液样本中的IL-10进行定量。脑膜炎球菌感染性休克患者的血清中显示出高水平的IL-10（平均为21,221 pg/ml；范围为25至64,500 pg/ml）。所有死亡病例的血清IL-10水平均≥1,000 pg/ml（平均为23,058 pg/ml；范围为1,000至64,500 pg/ml）。没有感染性休克的脑膜炎球菌性脑膜炎患者血清中的IL-10浓度相对较低（平均为119 pg/ml；范围为0至1,050 pg/ml），但脑脊液中IL-10呈现局部分泌。血清中IL-10的浓度与同一患者先前报告的血清中肿瘤坏死因子α、IL-6和IL-8的浓度呈正相关。我们得出结论，在脑膜炎球菌感染性休克的初始阶段，IL-10与促炎细胞因子一起被广泛激活，并且IL-10与脑膜炎球菌病的死亡相关。
