De Benedetti F, Massa M, Pignatti P, Albani S, Novick D, Martini A
Clinica Pediatrica, Universitá degli Studi di Pavia, Italy.
J Clin Invest. 1994 May;93(5):2114-9. doi: 10.1172/JCI117206.
By using a sandwich ELISA, soluble human IL-6 receptor (sIL-6 R) levels were measured in the sera of 20 healthy children and of 25 patients with systemic juvenile rheumatoid arthritis (JRA). In patients with systemic JRA, serum sIL-6 R levels (114.6 +/- 37.7 ng/ml) were significantly lower (P < 0.01) than those of healthy children (161.2 +/- 45.5 ng/ml). Serum sIL-6 R levels were negatively correlated (r = -0.610, P < 0.001) with serum IL-6 levels measured with the B9 cells. The serum IL-6/sIL-6 R complex was detected using an ELISA based on a monoclonal antibody to IL-6 for capture and on a monoclonal antibody to human sIL-6 R for detection. Healthy controls had little, if any, detectable serum IL-6/sIL-6 R complex (OD 0.024 +/- 0.027), while the majority of patients with systemic JRA presented measurable serum IL-6/sIL-6 R complex (OD 0.492 +/- 0.546). IL-6 levels estimated in the circulating IL-6/sIL-6 R complexes were in the range of nanograms per milliliter and approximately 20-fold higher than those measured by the B9 cells. Since serum C-reactive protein concentrations were much more correlated with serum levels of IL-6/sIL-6 R complexes (r = 0.713, r2 = 0.51, P < 0.0001) than with the serum IL-6 levels measured with the B9 cells (r = 0.435, r2 = 0.19, P = 0.05), the large quantities of serum IL-6 present in IL-6/sIL-6 R complexes appear to be biologically relevant in vivo, at least as far as the induction by IL-6 of acute phase protein production.
采用夹心酶联免疫吸附测定法（ELISA），检测了20名健康儿童和25名系统性幼年类风湿性关节炎（JRA）患者血清中的可溶性人白细胞介素6受体（sIL-6R）水平。在系统性JRA患者中，血清sIL-6R水平（114.6±37.7 ng/ml）显著低于健康儿童（161.2±45.5 ng/ml）（P<0.01）。血清sIL-6R水平与用B9细胞检测的血清白细胞介素6（IL-6）水平呈负相关（r=-0.610，P<0.001）。采用基于抗IL-6单克隆抗体捕获和抗人sIL-6R单克隆抗体检测的ELISA法检测血清IL-6/sIL-6R复合物。健康对照者血清中几乎检测不到IL-6/sIL-6R复合物（光密度0.024±0.027），而大多数系统性JRA患者血清中可检测到IL-6/sIL-6R复合物（光密度0.492±0.546）。循环中IL-6/sIL-6R复合物中估计的IL-6水平在每毫升纳克范围内，比用B9细胞检测的水平高约20倍。由于血清C反应蛋白浓度与血清IL-6/sIL-6R复合物水平（r=0.713，r2=0.51，P<0.0001）的相关性远高于与用B9细胞检测的血清IL-6水平（r=0.435，r2=0.19，P=0.05），因此IL-6/sIL-6R复合物中存在的大量血清IL-6在体内似乎具有生物学相关性，至少就IL-6诱导急性期蛋白产生而言。
