Gallo O, Gori A M, Attanasio M, Martini F, Giusti B, Boddi M, Gallina E, Fini O, Abbate R
Clinica Otorinolaringoiatrica, University of Florence, Italy.
Br J Cancer. 1993 Sep;68(3):465-8. doi: 10.1038/bjc.1993.371.
In patients with advanced head and neck squamous cell carcinoma (HNSC), evidence of cell-mediated immunity and monocyte functional abnormalities has been reported. We studied the production of interleukin 1 beta (IL-1 beta) and interleukin 6 (IL-6) by peripheral blood monocytes from 22 patients with HNSC (12 larynx and ten oral cavity cancers) in comparison with monocyte cytokine production of age-matched healthy subjects. Pure monocytes were incubated with and without lipopolysaccharides (LPS) (10 micrograms ml-1) for 4 h at 37 degrees C and IL-1 beta and IL-6 concentrations were determined in supernatants by specific ELISA. There was no significant difference in IL-1 beta levels in monocyte supernatants from cancer in comparison to control subjects; conversely, a higher IL-6 production by unstimulated and LPS-activated cells from HNSC patients than from controls was found. No relationship was observed between cytokine production and cancer stage. The regression analysis evidenced a significant correlation between IL-1 beta and IL-6 monocyte-release in HNSC patients and in controls, so suggesting a possible autocrine control of IL-6 production by other cytokines.
在晚期头颈部鳞状细胞癌（HNSC）患者中，已有细胞介导免疫和单核细胞功能异常的相关报道。我们研究了22例HNSC患者（12例喉癌和10例口腔癌）外周血单核细胞白细胞介素1β（IL-1β）和白细胞介素6（IL-6）的产生情况，并与年龄匹配的健康受试者的单核细胞细胞因子产生情况进行了比较。将纯化的单核细胞在有或无脂多糖（LPS）（10微克/毫升）的情况下于37℃孵育4小时，通过特异性酶联免疫吸附测定法（ELISA）测定上清液中IL-1β和IL-6的浓度。与对照受试者相比，癌症患者单核细胞上清液中的IL-1β水平无显著差异；相反，发现HNSC患者未受刺激和LPS激活的细胞产生的IL-6高于对照组。未观察到细胞因子产生与癌症分期之间的关系。回归分析表明，HNSC患者和对照者中IL-1β和IL-6单核细胞释放之间存在显著相关性，因此提示其他细胞因子可能对IL-6的产生存在自分泌控制。
