七种用于检测伯氏疏螺旋体抗体的商业试剂盒的比较。
Comparison of seven commercial kits for detection of antibodies to Borrelia burgdorferi.
作者信息
Schmitz J L, Powell C S, Folds J D
机构信息
Clinical Immunology Laboratory, University of North Carolina Hospitals, Chapel Hill 27514.
出版信息
Eur J Clin Microbiol Infect Dis. 1993 Jun;12(6):419-24. doi: 10.1007/BF01967435.
Five enzyme immunoassay (EIA) and two Western blot (WB) commercial kits were compared for their ability to detect antibodies to Borrelia burgdorferi. The panel of 53 test sera consisted of 25 sera positive for antibodies to Borrelia burgdorferi, 15 sera negative for such antibodies, 5 sera reactive in serologic tests for syphilis, and 8 sera containing antinuclear antibodies and/or rheumatoid factor. The rate of agreement with reference results was 93%, 90%, 90% and 88% for EIA kits from Diamedix, Cambridge Biotech, Mardx and Sigma respectively. The sensitivity and specificity was 84% and 100% respectively for Cambridge Biotech, 76% and 94% for Diamedix, 68% and 83% for Mardx, and 68% and 83% for Sigma. The three confirmatory tests, Cambridge Biotech WB, General Biometrics P39 EIA and Mardx WB, demonstrated 75%, 60% and 63% agreement respectively. The sensitivity and specificity was 52% and 100% respectively for Cambridge Biotech WB, 24% and 100% for General Biometrics P39 EIA, and 44% and 100% for Mardx WB. The results demonstrate the variable performance of commercial serologic kits for detection of antibodies to Borrelia burgdorferi. WB appears to be a better confirmatory test than the single protein EIA.
对五种酶免疫测定（EIA）和两种蛋白质印迹（WB）商业试剂盒检测伯氏疏螺旋体抗体的能力进行了比较。53份检测血清包括25份伯氏疏螺旋体抗体阳性血清、15份此类抗体阴性血清、5份梅毒血清学检测呈反应性的血清以及8份含有抗核抗体和/或类风湿因子的血清。Diamedix、剑桥生物技术公司、Mardx和西格玛公司的EIA试剂盒与参考结果的一致率分别为93%、90%、90%和88%。剑桥生物技术公司的灵敏度和特异性分别为84%和100%，Diamedix为76%和94%，Mardx为68%和83%，西格玛为68%和83%。三种确证试验，即剑桥生物技术公司的WB、通用生物计量学公司的P39 EIA和Mardx的WB，一致性分别为75%、60%和63%。剑桥生物技术公司WB的灵敏度和特异性分别为52%和100%，通用生物计量学公司P39 EIA为24%和100%，Mardx WB为44%和100%。结果表明，商业血清学试剂盒检测伯氏疏螺旋体抗体的性能各不相同。WB似乎是比单一蛋白EIA更好的确证试验。
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