Schimpl G, Pesendorfer P, Steinwender G, Feierl G, Ratschek M, Höllwarth M E
Department of Paediatric Surgery, University of Graz, Austria.
Gut. 1996 Jul;39(1):48-53. doi: 10.1136/gut.39.1.48.
Spontaneous bacterial infections and septicaemia result in morbidity and mortality in patients with portal hypertension and obstructive jaundice.
The aim of this study in rats was to investigate the incidence of bacterial translocation in portal hypertension and obstructive jaundice, and to evaluate the effects of allopurinol and glutamine.
Rats were subjected to sham laparotomy (SL), portal hypertension (PH) by calibrated stenosis of the portal vein, and common bile duct ligation (CBDL). Animals of each group were either treated with allopurinol (50 mg/kg twice a week), glutamine (1 g/kg/d), and allopurinol and glutamine.
After four weeks, significant bacterial translocation in the untreated PH and CBDL rats occurred. Intestinal mucosal malondialdehyde concentrations (MDA), as an indicator for lipid peroxidation, and myeloperoxidase activity (MPO) released from activated neutrophils were also significantly increased (p < 0.01). Allopurinol and glutamine in PH and CBDL rats improved bacterial translocation, and decreased MDA and MPO values (p < 0.01).
In PH and CBDL rats significant bacterial translocation, ileal mucosal lipid peroxidation, and neutrophil derived MPO activity occurred. Allopurinol and glutamine significantly reduced bacterial translocation, as well as ileal mucosal MDA and MPO activities.
自发性细菌感染和败血症会导致门静脉高压和梗阻性黄疸患者发病和死亡。
本研究在大鼠中进行，旨在调查门静脉高压和梗阻性黄疸时细菌移位的发生率，并评估别嘌呤醇和谷氨酰胺的作用。
大鼠接受假手术（SL）、通过门静脉校准狭窄造成门静脉高压（PH）以及胆总管结扎（CBDL）。每组动物分别用别嘌呤醇（50毫克/千克，每周两次）、谷氨酰胺（1克/千克/天）以及别嘌呤醇和谷氨酰胺进行治疗。
四周后，未治疗的PH和CBDL大鼠发生了显著的细菌移位。作为脂质过氧化指标的肠黏膜丙二醛浓度（MDA）以及活化中性粒细胞释放的髓过氧化物酶活性（MPO）也显著增加（p<0.01）。PH和CBDL大鼠中的别嘌呤醇和谷氨酰胺改善了细菌移位，并降低了MDA和MPO值（p<0.01）。
在PH和CBDL大鼠中发生了显著的细菌移位、回肠黏膜脂质过氧化以及中性粒细胞衍生的MPO活性。别嘌呤醇和谷氨酰胺显著减少了细菌移位以及回肠黏膜MDA和MPO活性。
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