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Species differentiation by internally transcribed spacer PCR and HhaI digestion of fluconazole-resistant Candida krusei, Candida inconspicua, and Candida norvegensis strains.通过内部转录间隔区PCR和HhaI酶切对氟康唑耐药的克鲁斯念珠菌、隐匿念珠菌和挪威念珠菌菌株进行菌种鉴别。
J Clin Microbiol. 1997 Apr;35(4):1036-9. doi: 10.1128/jcm.35.4.1036-1039.1997.
2
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Restriction enzyme analysis of ribosomal DNA shows that Candida inconspicua clinical isolates can be misidentified as Candida norvegensis with traditional diagnostic procedures.核糖体DNA的限制性内切酶分析表明，使用传统诊断方法， inconspicua念珠菌临床分离株可能会被误鉴定为挪威念珠菌。
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本文引用的文献

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New and emerging yeast pathogens.新型及新兴酵母病原体
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Karyotyping of fluconazole-resistant yeasts with phenotype reported as Candida krusei or Candida inconspicua.对表型报告为克柔念珠菌或隐匿念珠菌的氟康唑耐药酵母进行核型分析。
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Rapid presumptive identification of medically relevant yeasts to the species level by polymerase chain reaction and restriction enzyme analysis.通过聚合酶链反应和限制性酶切分析对医学相关酵母进行快速的种属水平初步鉴定。
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Specific identification of Candida albicans by hybridization with oligonucleotides derived from ribosomal DNA internal spacers.通过与源自核糖体DNA内部间隔区的寡核苷酸杂交对白色念珠菌进行特异性鉴定。
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Candida krusei: biology, epidemiology, pathogenicity and clinical manifestations of an emerging pathogen.克鲁斯念珠菌：一种新兴病原体的生物学、流行病学、致病性及临床表现
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Microtitration plate enzyme immunoassay to detect PCR-amplified DNA from Candida species in blood.微量滴定板酶免疫测定法检测血液中念珠菌属经聚合酶链反应扩增的DNA。
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Detection of amphotericin B-resistant Candida isolates in a broth-based system.在基于肉汤的系统中检测对两性霉素B耐药的念珠菌分离株。
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通过内部转录间隔区PCR和HhaI酶切对氟康唑耐药的克鲁斯念珠菌、隐匿念珠菌和挪威念珠菌菌株进行菌种鉴别。

Species differentiation by internally transcribed spacer PCR and HhaI digestion of fluconazole-resistant Candida krusei, Candida inconspicua, and Candida norvegensis strains.

作者信息

Nho S, Anderson M J, Moore C B, Denning D W

机构信息

Department of Medicine, Hope Hospital, Salford, United Kingdom.

出版信息

J Clin Microbiol. 1997 Apr;35(4):1036-9. doi: 10.1128/jcm.35.4.1036-1039.1997.

DOI:10.1128/jcm.35.4.1036-1039.1997
PMID:9157129
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC229734/
Abstract

PCR amplification of the regions containing the internally transcribed spacers and 5.8S rRNA gene of Candida krusei, C. inconspicua, and C. norvegensis yielded fragments of 510, 460, and 500 bp, respectively. HhaI digestion of these fragments yielded species-specific bands. Random amplification of polymorphic DNA with primer R108 showed interspecific discriminatory band patterns. Susceptibilities to fluconazole and amphotericin B were determined.

摘要

对包含克鲁斯念珠菌、隐匿念珠菌和挪威念珠菌的内转录间隔区和5.8S rRNA基因的区域进行PCR扩增，分别产生了510、460和500 bp的片段。用HhaI对这些片段进行酶切产生了物种特异性条带。用引物R108进行随机扩增多态性DNA显示出种间鉴别条带模式。测定了对氟康唑和两性霉素B的敏感性。