通过内部转录间隔区PCR和HhaI酶切对氟康唑耐药的克鲁斯念珠菌、隐匿念珠菌和挪威念珠菌菌株进行菌种鉴别。
Species differentiation by internally transcribed spacer PCR and HhaI digestion of fluconazole-resistant Candida krusei, Candida inconspicua, and Candida norvegensis strains.
作者信息
Nho S, Anderson M J, Moore C B, Denning D W
机构信息
Department of Medicine, Hope Hospital, Salford, United Kingdom.
出版信息
J Clin Microbiol. 1997 Apr;35(4):1036-9. doi: 10.1128/jcm.35.4.1036-1039.1997.
Abstract
PCR amplification of the regions containing the internally transcribed spacers and 5.8S rRNA gene of Candida krusei, C. inconspicua, and C. norvegensis yielded fragments of 510, 460, and 500 bp, respectively. HhaI digestion of these fragments yielded species-specific bands. Random amplification of polymorphic DNA with primer R108 showed interspecific discriminatory band patterns. Susceptibilities to fluconazole and amphotericin B were determined.
摘要
对包含克鲁斯念珠菌、隐匿念珠菌和挪威念珠菌的内转录间隔区和5.8S rRNA基因的区域进行PCR扩增，分别产生了510、460和500 bp的片段。用HhaI对这些片段进行酶切产生了物种特异性条带。用引物R108进行随机扩增多态性DNA显示出种间鉴别条带模式。测定了对氟康唑和两性霉素B的敏感性。
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