Essevaz-Roulet B, Bockelmann U, Heslot F
Laboratoire de Physique de la Matière Condensée, URA 1437, Ecole Normale Supérieure, 24 rue Lhomond, 75005 Paris, France.
Proc Natl Acad Sci U S A. 1997 Oct 28;94(22):11935-40. doi: 10.1073/pnas.94.22.11935.
We describe the mechanical separation of the two complementary strands of a single molecule of bacteriophage lambda DNA. The 3' and 5' extremities on one end of the molecule are pulled progressively apart, and this leads to the opening of the double helix. The typical forces along the opening are in the range of 10-15 pN. The separation force signal is shown to be related to the local GC vs. AT content along the molecule. Variations of this content on a typical scale of 100-500 bases are presently detected.
我们描述了噬菌体λDNA单分子两条互补链的机械分离过程。分子一端的3'和5'末端被逐渐拉开，这导致双螺旋结构打开。沿打开方向的典型力在10 - 15皮牛的范围内。分离力信号显示与分子上局部的GC与AT含量有关。目前已检测到该含量在100 - 500个碱基的典型尺度上的变化。
