Anant S, Roy S, VijayRaghavan K
National Centre for Biological Sciences, Bangalore, India.
Development. 1998 Apr;125(8):1361-9. doi: 10.1242/dev.125.8.1361.
Twist is required in Drosophila embryogenesis for mesodermal specification and cell-fate choice. We have examined the role of Twist and Notch during adult indirect flight muscle development. Reduction in levels of Twist leads to abnormal myogenesis. Notch reduction causes a similar mutant phenotype and reduces Twist levels. Conversely, persistent expression, in myoblasts, of activated Notch causes continued twist expression and failure of differentiation as assayed by myosin expression. The gain-of-function phenotype of Notch is very similar to that seen upon persistent twist expression. These results point to a relationship between Notch function and twist regulation during indirect flight muscle development and show that decline in Twist levels is a requirement for the differentiation of these muscles, unlike the somatic muscles of the embryo.
在果蝇胚胎发育过程中，Twist对于中胚层特化和细胞命运选择是必需的。我们研究了Twist和Notch在成年果蝇间接飞行肌发育过程中的作用。Twist水平降低会导致肌生成异常。Notch水平降低会导致类似的突变表型，并降低Twist水平。相反，在成肌细胞中持续表达激活的Notch会导致Twist持续表达，并且如通过肌球蛋白表达所检测的那样，会导致分化失败。Notch的功能获得型表型与持续表达Twist时所观察到的表型非常相似。这些结果表明在间接飞行肌发育过程中Notch功能与Twist调节之间存在关联，并且表明与胚胎的体肌不同，Twist水平的下降是这些肌肉分化的必要条件。
