使用累积偏斜图分析基因组。
Analyzing genomes with cumulative skew diagrams.
作者信息
Grigoriev A
机构信息
Max-Planck-Institute for Molecular Genetics, Ihnestrasse 73, 14195 Berlin, Germany.
出版信息
Nucleic Acids Res. 1998 May 15;26(10):2286-90. doi: 10.1093/nar/26.10.2286.
Abstract
A novel method of cumulative diagrams shows that the nucleotide composition of a microbial chromosome changes at two points separated by about a half of its length. These points coincide with sites of replication origin and terminus for all bacteria where such sites are known. The leading strand is found to contain more guanine than cytosine residues. This fact is used to predict origin and terminus locations in other bacterial and archaeal genomes. Local changes, visible as diagram distortions, may represent recent genome rearrangements, as demonstrated for two strains of Escherichia coli . Analysis of the diagrams of viral and mitochondrial genomes suggests a link between the base composition bias and the time spent by DNA in a single stranded state during replication.
摘要
一种新的累积图方法表明，微生物染色体的核苷酸组成在两个相隔约其长度一半的点处发生变化。对于所有已知复制起点和终点位置的细菌，这些点与复制起点和终点的位置重合。发现前导链所含鸟嘌呤残基比胞嘧啶残基多。这一事实被用于预测其他细菌和古细菌基因组中的起点和终点位置。如图形扭曲所示的局部变化可能代表最近的基因组重排，这在两株大肠杆菌中得到了证明。对病毒和线粒体基因组图谱的分析表明，碱基组成偏差与DNA在复制过程中处于单链状态的时间之间存在联系。
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