对大肠杆菌K-12中一个琥珀突变的遗传分析，该突变影响脱氧核糖核酸连接酶和生存能力。

Genetic analyses of an amber mutation in Escherichia coli K-12, affecting deoxyribonucleic acid ligase and viability.

作者信息

Sato T, Horiuchi T, Nagata T

出版信息

J Bacteriol. 1975 Dec;124(3):1089-96. doi: 10.1128/jb.124.3.1089-1096.1975.

DOI:10.1128/jb.124.3.1089-1096.1975

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC236010/

Abstract

Genetic analyses of an Escherichia coli K-12 mutant possessing the amber mutation lig-321 were carried out. This mutant is defective in deoxyribonucleic acid (DNA) ligase and conditionally lethal. We constructed strains harboring an F'lig+ or F'lig-321 plasmid. Genetic complementation analyses were done by using these plasmids and by constructing a lig-4/F'lig-321 merodiploid. It was shown that lig-321 does not complement lig-4, unless the former is suppressed by an amber suppressor. The same was found to be the case between lig-321 and lig-ts7. Transductional mapping of lig-321, by a four-factor cross, revealed that lig-321 is very closely linked to lig-4. The frequency of recombinants between the two alleles was not unreasonable for assuming that they arose by intragenic recombination. The lig-4 and lig-ts7 alleles are known to reside in the structural gene for DNA ligase, in which lig-321 may also be located.

摘要

对具有琥珀突变体lig-321的大肠杆菌K-12突变体进行了遗传分析。该突变体的脱氧核糖核酸（DNA）连接酶存在缺陷且具有条件致死性。我们构建了携带F'lig+或F'lig-321质粒的菌株。通过使用这些质粒并构建lig-4/F'lig-321部分二倍体进行了遗传互补分析。结果表明，除非lig-321被琥珀抑制子抑制，否则它不能互补lig-4。在lig-321和lig-ts7之间也发现了同样的情况。通过四因子杂交对lig-321进行转导作图，结果显示lig-321与lig-4紧密连锁。两个等位基因之间的重组频率对于假设它们通过基因内重组产生来说并非不合理。已知lig-4和lig-ts7等位基因位于DNA连接酶的结构基因中，lig-321可能也位于该结构基因中。

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