Cardoso Olga, Leitão Rui, Figueiredo Alexandra, Sousa João Carlos, Duarte Aida, Peixe Luísa Vieira
Laboratório de Microbiologia C.E.F. da Faculdade de Farmácia da Universidade de Coimbra, Portugal.
Microb Drug Resist. 2002 Summer;8(2):93-7. doi: 10.1089/107662902760190635.
Resistance to carbapenems is emerging, and it is a great problem to therapeutics. Three isolates of Pseudomonas aeruginosa from a Portuguese hospital identified in urine and sputum, in 1995, presented a high-level resistance to imipenem (> 32 mg/L). Afterward, one isolate of P. aeruginosa recovered from urine of an ambulatory patient in 1998 showed high resistance to imipenem and meropenem. The resistance to carbapenems in these strains was associated with the production of a class B beta-lactamase, as was demonstrated by imipenem hydrolysis and inhibition by EDTA. Using primers described for bla(IMP) and bla(VIM), the amplification of the latter was observed in all isolates and a VIM-2 metallo-enzyme was identified. The pulsed-field gel electrophoresis (PFGE) patterns of these isolates were indistinguishable, suggesting dissemination to the community of this VIM-2 producer.
对碳青霉烯类药物的耐药性正在出现，这对治疗来说是个大问题。1995年从一家葡萄牙医院的尿液和痰液中分离出的三株铜绿假单胞菌对亚胺培南呈现出高水平耐药（>32 mg/L）。之后，1998年从一名门诊患者尿液中分离出的一株铜绿假单胞菌对亚胺培南和美罗培南表现出高度耐药。这些菌株对碳青霉烯类药物的耐药性与一种B类β-内酰胺酶的产生有关，亚胺培南水解和EDTA抑制实验证实了这一点。使用针对bla(IMP)和bla(VIM)描述的引物，在所有分离株中均观察到后者的扩增，并鉴定出一种VIM-2金属酶。这些分离株的脉冲场凝胶电泳（PFGE）图谱无法区分，表明这种VIM-2产生菌已传播至社区。
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