Cordova Amado, Deserno Markus, Gelbart William M, Ben-Shaul Avinoam
Department of Chemistry and Biochemistry, University of California Los Angeles, Los Angeles, California 90095-1569, USA.
Biophys J. 2003 Jul;85(1):70-4. doi: 10.1016/S0006-3495(03)74455-5.
Osmotic shock is a familiar means for rupturing viral capsids and exposing their genomes intact. The necessary conditions for providing this shock involve incubation in high-concentration salt solutions, and lower permeability of the capsids to salt ions than to water molecules. We discuss here how values of the capsid strength can be inferred from calculations of the osmotic pressure differences associated with measured values of the critical concentration of incubation solution.
渗透压休克是一种常见的使病毒衣壳破裂并完整暴露其基因组的方法。产生这种休克的必要条件包括在高浓度盐溶液中孵育，以及衣壳对盐离子的通透性低于对水分子的通透性。我们在此讨论如何通过与孵育溶液临界浓度的测量值相关的渗透压差异计算来推断衣壳强度值。
