Nicolelis Miguel A L, Dimitrov Dragan, Carmena Jose M, Crist Roy, Lehew Gary, Kralik Jerald D, Wise Steven P
Department of Neurobiology, Duke University, Durham, NC 27710, USA.
Proc Natl Acad Sci U S A. 2003 Sep 16;100(19):11041-6. doi: 10.1073/pnas.1934665100. Epub 2003 Sep 5.
A paradigm is described for recording the activity of single cortical neurons from awake, behaving macaque monkeys. Its unique features include high-density microwire arrays and multichannel instrumentation. Three adult rhesus monkeys received microwire array implants, totaling 96-704 microwires per subject, in up to five cortical areas, sometimes bilaterally. Recordings 3-4 weeks after implantation yielded 421 single neurons with a mean peak-to-peak voltage of 115 +/- 3 microV and a signal-to-noise ratio of better than 5:1. As many as 247 cortical neurons were recorded in one session, and at least 58 neurons were isolated from one subject 18 months after implantation. This method should benefit neurophysiological investigation of learning, perception, and sensorimotor integration in primates and the development of neuroprosthetic devices.
本文描述了一种用于记录清醒、行为猕猴单个皮层神经元活动的范式。其独特之处包括高密度微丝阵列和多通道仪器。三只成年恒河猴接受了微丝阵列植入，每只猴子在多达五个皮层区域植入，有时是双侧植入，每个区域植入96 - 704根微丝。植入后3 - 4周的记录获得了421个单个神经元，其平均峰峰值电压为115 +/- 3微伏，信噪比大于5:1。在一次记录中最多可记录247个皮层神经元，在植入18个月后，从一只猴子身上至少分离出58个神经元。这种方法将有助于对灵长类动物的学习、感知和感觉运动整合进行神经生理学研究，以及神经假体装置的开发。
